Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity.
Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity.
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DOI：
10.1007/s00253-008-1525-z
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发表时间：
2008-08
影响因子：
5
通讯作者：
Mai C
中科院分区：
文献类型：
--
作者：
Verma P;Dyckmans J;Militz H;Mai C
用1，3-二羟甲基-4，5-二羟基乙烯脲（DMDHEU）处理山毛榉和松木块，以增加增重百分比（WPG）。处理后的标本对变色栓菌和灰霉病菌的抗性，测定为质量损失，随着DMDHEU WPG的增加而增加。木块中真菌的代谢活性被评估为基于荧光素二乙酸酯水解的总酯酶活性（TEA）和通过等温微量热法测定的热量或能量产生。两种方法均表明，真菌活性与WPG和真菌引起的质量损失有关。尽管如此，即使在最高WPG的木块中也检测到真菌活性，并表明该处理对真菌无毒。与TEA相比，能量产生与衰变后质量损失的一致性更高;更高的质量损失更严格地反映在更高的产热速率上。产热没有线性进行，可能是由于过量的二氧化碳抑制真菌的活性。
Beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen urea (DMDHEU) to increasing weight percent gains (WPG). The resistance of the treated specimens against Trametes versicolor and Coniophora puteana, determined as mass loss, increased with increasing WPG of DMDHEU. Metabolic activity of the fungi in the wood blocks was assessed as total esterase activity (TEA) based on the hydrolysis of fluorescein diacetate and as heat or energy production determined by isothermal micro-calorimetry. Both methods revealed that the fungal activity was related with the WPG and the mass loss caused by the fungi. Still, fungal activity was detected even in wood blocks of the highest WPG and showed that the treatment was not toxic to the fungi. Energy production showed a higher consistency with the mass loss after decay than TEA; higher mass loss was more stringently reflected by higher heat production rate. Heat production did not proceed linearly, possibly due to the inhibition of fungal activity by an excess of carbon dioxide.
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影响因子：
4.4
作者：
KEREM, Z;FRIESEM, D;HADAR, Y
通讯作者：
HADAR, Y
影响因子：
3.4
作者：
Donath, S;Militz, H;Mai, C
通讯作者：
Mai, C
DOI：
10.1016/j.ibiod.2004.07.003
发表时间：
2005-01-01
影响因子：
4.8
作者：
Hill, CAS;Forster, SC;Williams, GR
通讯作者：
Williams, GR
影响因子：
3.2
作者：
DERMOUN, Z;BELAICH, JP
通讯作者：
BELAICH, JP
DOI：
10.1007/bf02607895
发表时间：
1991-04-01
期刊：
HOLZ ALS ROH-UND WERKSTOFF
影响因子：
--
作者：
MILITZ, H
通讯作者：
MILITZ, H