Spatial rearrangement and enhanced clustering of kinetochores in interphase nuclei of dorsal root ganglion neurons in vitro: association with nucleolar fusion.
Spatial rearrangement and enhanced clustering of kinetochores in interphase nuclei of dorsal root ganglion neurons in vitro: association with nucleolar fusion.
复制标题
体外背根神经节神经元间期核的空间重排和增强的动粒聚集：与核仁融合的关联。
DOI：
10.1016/0014-4827(92)90058-g
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发表时间：
1992
影响因子：
3.7
通讯作者：
U. Boni
中科院分区：
文献类型：
--
作者：
P. Park;U. Boni
几种细胞类型的间期核显示出独特的，非随机排列的特定染色质结构域。有人认为，这种安排是与细胞的功能承诺，并从特定的DNA序列的转录活性位点区室化的结果。在一个测试的假设，这种拓扑组织是建立在细胞分化过程中，着丝粒的空间分布测定，在背根神经节神经元体外，使用免疫细胞化学与荧光显微镜，共聚焦激光显微镜，和超微结构的免疫金技术。着丝粒以簇的形式出现，与核仁和核膜相关联。神经元在不同阶段的分化，确定核仁分布，表现出一个独特的，阶段特异性，空间组织的动粒。形态计量学分析，连同串行重建，表明，渐进聚类的着丝粒伴随分化，这种聚类发生在与核仁融合。所提供的数据表明，在完全分化状态下观察到的染色质组织可能是在分化过程中特定染色质结构域的受控重排的结果，并且这种重排的机制和细胞分化的过程可能是相关的。
Interphase nuclei of several cell types display distinct, nonrandom arrangements of specific chromatin domains. It has been suggested that this arrangement is associated with the functional commitment of the cell and results from compartmentalization of specific DNA sequences to transcriptionally competent sites. In a test of the hypothesis that such topological organization is established during cellular differentiation, the spatial distribution of centromeres was determined, in dorsal root ganglion neuronsin vitro, using immunocytochemistry in conjunction with fluorescence microscopy, confocal laser microscopy, and ultrastructural immuno-gold techniques. Kinetochores occurred as clusters, in association with nucleoli and with the nuclear envelope. Neurons at different stages of differentiation, as determined by nucleolar distribution, exhibited a distinct, stage-specific, spatial organization of kinetochores. Morphometric analyses, together with serial reconstruction, indicated that progressive clustering of kinetochores accompanies differentiation and that such clustering occurs in association with nucleolar fusion. The data presented indicate that the chromatin organization observed in the fully differentiated state may be the result of controlled rearrangements of specific chromatin domains during differentiation and that the mechanism governing such rearrangement and the process of cellular differentiation may be linked.
DOI：
10.1016/s0021-9258(17)38689-1
发表时间：
1985-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Greenberg;L. Greene;E. Ziff
通讯作者：
M. Greenberg;L. Greene;E. Ziff
影响因子：
56.9
作者：
BORDEN, J;MANUELIDIS, L
通讯作者：
MANUELIDIS, L
影响因子：
56.9
作者：
GREENBERG, ME;ZIFF, EB;GREENE, LA
通讯作者：
GREENE, LA