Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses.
Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses.
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甘薯谷胱甘肽 S-转移酶基因家族的鉴定和分析揭示了地上和地下组织中响应非生物胁迫的不同 GST 介导的网络
DOI：
10.1186/s12870-017-1179-z
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发表时间：
2017-11-28
影响因子：
5.3
通讯作者：
Luo Y
中科院分区：
文献类型：
--
作者：
Ding N;Wang A;Zhang X;Wu Y;Wang R;Cui H;Huang R;Luo Y
甘薯是一种缺乏参考基因组的六倍体物种，是许多发展中国家最重要的农作物之一，在这些国家，非生物胁迫是导致作物减产的主要原因。谷胱甘肽S转移酶(GST)是一种多功能酶，在氧化应激耐受和细胞解毒中发挥重要作用。结果从两个本地转录组数据库中克隆了42个GST全长基因，并通过分子克隆和Sanger测序进行了验证。序列和种内系统发育分析表明，它们在编码序列上存在着广泛的分化，并将它们划分为8个亚家族。种间系统发育和比较分析表明，所研究的大多数GST同源基因可能在甘薯物种形成之前起源和分化。大规模RNA-SEQ和实时定量聚合酶链式反应实验的结果显示，不同组织和品种的基因表达谱存在着广泛的差异，这意味着它们在基因表达调控方面存在着强烈的进化差异。此外，我们还进行了5个操纵胁迫实验，揭示了甘薯GST基因在地上和地下组织中的胁迫响应模式。结论本研究发现了大量甘薯GST基因，系统地研究了它们的进化多样性，为研究甘薯GST介导的胁迫响应机制提供了新的见解。
BackgroundSweet potato, a hexaploid species lacking a reference genome, is one of the most important crops in many developing countries, where abiotic stresses are a primary cause of reduction of crop yield. Glutathione S-transferases (GSTs) are multifunctional enzymes that play important roles in oxidative stress tolerance and cellular detoxification.ResultsA total of 42 putative full-length GST genes were identified from two local transcriptome databases and validated by molecular cloning and Sanger sequencing. Sequence and intraspecific phylogenetic analyses revealed extensive differentiation in their coding sequences and divided them into eight subfamilies. Interspecific phylogenetic and comparative analyses indicated that most examined GST paralogs might originate and diverge before the speciation of sweet potato. Results from large-scale RNA-seq and quantitative real-time PCR experiments exhibited extensive variation in gene-expression profiles across different tissues and varieties, which implied strong evolutionary divergence in their gene-expression regulation. Moreover, we performed five manipulated stress experiments and uncovered highly divergent stress-response patterns of sweet potato GST genes in aboveground and underground tissues.ConclusionsOur study identified a large number of sweet potato GST genes, systematically investigated their evolutionary diversification, and provides new insights into the GST-mediated stress-response mechanisms in this worldwide crop.
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