Phosphokinase antibody arrays on dendron-coated surface.
Phosphokinase antibody arrays on dendron-coated surface.
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DOI：
10.1371/journal.pone.0096456
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Park JW
中科院分区：
文献类型：
--
作者：
Kwak JW;Jeong H;Han SH;Kim Y;Son SM;Mook-Jung I;Hwang D;Park JW
在细胞水平上监测蛋白质磷酸化对于理解细胞内信号传导是重要的。在磷酸化蛋白质组学方法中，磷酸激酶抗体阵列已成为测量胞内信号中充分表征的磷酸化的优选工具。在这里，我们提出了一个树枝状涂层磷酸激酶抗体阵列（DPA），其中抗体被固定在树枝状涂层的载玻片上。在尺寸和结构上得到良好控制的圆锥形树突的自组装导致固定化的磷酸位点特异性抗体之间的精确控制的横向间距，从而使空间位阻最小化并改善抗原-抗体结合动力学。这些特征增加了蛋白磷酸化测量量的灵敏度、选择性和再现性。为了证明DPA的实用性，我们生成了从阿尔茨海默病（AD）模型小鼠获得的脑组织样品的磷酸化谱。分析的配置文件显示信号通路失调的过程中，AD的进展。
Monitoring protein phosphorylation at the cellular level is important to understand the intracellular signaling. Among the phosphoproteomics methods, phosphokinase antibody arrays have emerged as preferred tools to measure well-characterized phosphorylation in the intracellular signaling. Here, we present a dendron-coated phosphokinase antibody array (DPA) in which the antibodies are immobilized on a dendron-coated glass slide. Self-assembly of conically shaped dendrons well-controlled in size and structure resulted in precisely controlled lateral spacing between the immobilized phosphosite-specific antibodies, leading to minimized steric hindrance and improved antigen-antibody binding kinetics. These features increased sensitivity, selectivity, and reproducibility in measured amounts of protein phosphorylation. To demonstrate the utility of the DPA, we generated the phosphorylation profiles of brain tissue samples obtained from Alzheimer's disease (AD) model mice. The analysis of the profiles revealed signaling pathways deregulated during the course of AD progression.
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影响因子：
7
作者：
Cutillas, PR;Geering, B;Waterfield, MD
通讯作者：
Waterfield, MD
影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
14.9
作者：
Chatr-Aryamontri A;Breitkreutz BJ;Heinicke S;Boucher L;Winter A;Stark C;Nixon J;Ramage L;Kolas N;O'Donnell L;Reguly T;Breitkreutz A;Sellam A;Chen D;Chang C;Rust J;Livstone M;Oughtred R;Dolinski K;Tyers M
通讯作者：
Tyers M
影响因子：
14.9
作者：
Hornbeck PV;Kornhauser JM;Tkachev S;Zhang B;Skrzypek E;Murray B;Latham V;Sullivan M
通讯作者：
Sullivan M
DOI：
10.1073/pnas.220413597
发表时间：
2000-10-24
影响因子：
11.1
作者：
Fang, XJ;Yu, SX;Mills, GB
通讯作者：
Mills, GB