A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways.
A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response of Arabidopsis to salt stress via multiple signaling pathways.
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从西瓜中分离出的热休克 20 蛋白 (ClHSP22.8) 通过多种信号通路负调节拟南芥对盐胁迫的反应。
作者：
He Y;Yao Y;Li L;Li Y;Gao J;Fan M
热休克蛋白20（HSP 20）最初被证明在热休克胁迫过程中发挥作用，然而，最近的数据表明，HSP 20蛋白也参与植物的非生物胁迫。西瓜是众所周知的，是容易受到各种压力，然而，热休克蛋白20蛋白还没有被调查，并在西瓜的特点。在以前的研究中，我们确定了西瓜盐胁迫反应的负调节因子：ClHSP 22.8，HSP 20家族的成员。实时荧光定量PCR（qRT-PCR）和启动子：：β-葡萄糖醛酸酶（GUS）分析表明，ClHSP 22. 8在西瓜的不同组织中广泛表达，尤其是在7天龄幼苗和花的根部。qRT-PCR和GUS染色结果表明，外源脱落酸（阿坝）和盐胁迫显著抑制了ClHSP 22. 8的表达。ClHSP 22.8在拟南芥中的过表达导致对阿坝的敏感性和对盐胁迫的耐受性降低。此外，与ABA依赖性和非依赖性途径以及其他应激反应信号传导途径相关的关键调节因子的表达模式也在过表达ClHSP 22.8的转基因系中受到抑制。这些结果表明，ClHSP 22.8是植物响应盐胁迫的负调节因子，并通过ABA依赖和非依赖以及其他胁迫响应信号途径发生。
Heat-shock protein 20s (HSP20) were initially shown to play a role during heat shock stress; however, recent data indicated that HSP20 proteins are also involved in abiotic stress in plants. Watermelon is known to be vulnerable to various stressors; however, HSP20 proteins have yet to be investigated and characterized in the watermelon. In a previous study, we identified a negative regulator of salt stress response from watermelon: ClHSP22.8, a member of the HSP20 family. Quantitative real-time PCR (qRT-PCR) and promoter::β-glucuronidase (GUS) analysis revealed that ClHSP22.8 was expressed widely in a range of different tissues from the watermelon, but particularly in the roots of 7-day-old seedlings and flowers. Furthermore, qRT-PCR and GUS staining showed that the expression of ClHSP22.8 was significantly repressed by exogenous abscisic acid (ABA) and salt stress. The over-expression of ClHSP22.8 in Arabidopsis lines resulted in hypersensitivity to ABA and reduced tolerance to salt stress. Furthermore, the expression patterns of key regulators associated with ABA-dependent and independent pathways, and other stress-responsive signaling pathways, were also repressed in transgenic lines that over-expressed ClHSP22.8. These results indicated that ClHSP22.8 is a negative regulator in plant response to salt stress and occurs via ABA-dependent and independent, and other stress-responsive signaling pathways.
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