Involvement of multiple phytoene synthase genes in tissue- and cultivar-specific accumulation of carotenoids in loquat.
Involvement of multiple phytoene synthase genes in tissue- and cultivar-specific accumulation of carotenoids in loquat.
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DOI：
10.1093/jxb/eru257
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发表时间：
2014-08
影响因子：
6.9
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Fu X;Feng C;Wang C;Yin X;Lu P;Grierson D;Xu C;Chen K
对四个八氢番茄红素合酶基因和几个变体进行了表征，并确定了它们在白肉和红肉枇杷叶、果皮和果肉中类胡萝卜素积累差异中的进化和功能。在植物中，组织和品种之间类胡萝卜素积累的差异很常见。白肉枇杷品种的果肉中类胡萝卜素含量较低，但成熟后果皮中类胡萝卜素积累，叶子中积累的类胡萝卜素与红肉枇杷品种相似。分析了四个八氢番茄红素合酶 (PSY) 基因 EjPSY1、EjPSY2A、EjPSY2B 和 EjPSY3 的催化活性和表达模式，以了解它们在不同枇杷 (Eriobotrya japonica Lindl.) 类型中的作用。 EjPSY1 负责果皮而非果肉中类胡萝卜素的合成，而 EjPSY2A 负责成熟果实果肉中类胡萝卜素的积累。在白肉品种中发现了一个突变体EjPSY2A d，其组织特异性和表达水平与EjPSY2A相同，但缺乏C端区域和相应的催化活性，这解释了白肉品种中类胡萝卜素的缺乏。 EjPSY2B 的催化作用在叶子中最为显着。 EjPSY1和EjPSY2B的组织特异性表达很好地解释了在失去功能性EjPSY2A的白肉品种中，果皮和叶组织如何仍然积累类胡萝卜素。在所有检查的组织中，EjPSY3 mRNA 丰度比其他 PSY mRNA 丰度低约 1000 倍。此外，正常大小的转录物和两种选择性剪接形式（LYQ 中的 EjPSY3α 和 BS 品种中的 EjPSY3β）均不编码功能酶，因此得出结论 EjPSY3 在类胡萝卜素积累中不起任何作用。此外，值得注意的是，PSY 基因的募集在不同植物的特定组织中表达是独立于基因结构和进化起源而发生的。
Four phytoene synthase genes and several variants were characterized, and their evolution and function in differential carotenoid accumulation in leaf, peel, and flesh of white- and red-fleshed loquats were established. Differences in carotenoid accumulation between tissues and cultivars is common in plants. White-fleshed loquat cultivars had low levels of carotenoids in the flesh, but accumulated carotenoids in peel when ripe, and the leaves accumulated similar carotenoids to those in the red-fleshed loquat cultivars. The catalytic activity and expression patterns of four phytoene synthase (PSY) genes, EjPSY1, EjPSY2A, EjPSY2B, and EjPSY3, were analysed to understand their roles in different loquat (Eriobotrya japonica Lindl.) types. EjPSY1 was responsible for carotenoid synthesis in the fruit peel but not the flesh, whereas EjPSY2A was responsible for carotenoid accumulation in flesh of ripening fruit. A mutant EjPSY2A d, with the same tissue specificity and expression level as EjPSY2A, but lacking the C-terminal region and corresponding catalytic activity, was discovered in white-fleshed varieties, explaining the lack of carotenoids in the white flesh. The catalytic role of EjPSY2B was most significant in leaves. The tissue-specific expression of EjPSY1 and EjPSY2B explained well how peel and leaf tissues can still accumulate carotenoids in white-fleshed cultivars, which have lost the functional EjPSY2A. EjPSY3 mRNA abundance was ~1000-fold less than that of other PSY mRNAs in all tissues examined. In addition, neither the normal sized transcript nor two alternatively spliced forms, EjPSY3α in LYQ and EjPSY3β in BS cultivars, encoded functional enzymes, and it is concluded that EjPSY3 plays no role in carotenoid accumulation. In addition, it was noted that recruitment of PSY genes for expression in specific tissues of different plants has occurred independently of gene structure and evolutionary origin.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
7.4
作者：
Fantini, Elio;Falcone, Giulia;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
影响因子：
5.1
作者：
Fraser, PD;Kiano, JW;Bramley, PM
通讯作者：
Bramley, PM
影响因子：
7.2
作者：
Bai, Chao;Rivera, Sol M.;Zhu, Changfu
通讯作者：
Zhu, Changfu
影响因子：
7.3
作者：
Howitt, CA;Pogson, BJ
通讯作者：
Pogson, BJ