Genome-wide characterization of methylguanosine-capped and polyadenylated small RNAs in the rice blast fungus Magnaporthe oryzae.
Genome-wide characterization of methylguanosine-capped and polyadenylated small RNAs in the rice blast fungus Magnaporthe oryzae.
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DOI：
10.1093/nar/gkq583
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发表时间：
2010-11
影响因子：
14.9
通讯作者：
Dean RA
中科院分区：
文献类型：
--
作者：
Gowda M;Nunes CC;Sailsbery J;Xue M;Chen F;Nelson CA;Brown DE;Oh Y;Meng S;Mitchell T;Hagedorn CH;Dean RA
小rna在高等真核生物如哺乳动物和植物中有很好的描述；然而，对丝状真菌等简单真核生物的了解有限。本研究通过对稻瘟病菌Magnaporthe oryzae的差异RNA选择、全长cDNA克隆和454转录组测序，发现并鉴定了甲基鸟苷帽化和聚腺苷化的小RNA （CPA-sRNAs）。这种真菌会导致稻瘟病，这是一种毁灭性的疾病，稻瘟病是世界上一半以上人口的主要食物。CPA-sRNAs主要定位于蛋白质编码基因的转录起始和终止位点，并与基因表达呈正相关，特别是对于编码核糖体蛋白的高表达基因。许多CPA-sRNAs也映射到rnas、tRNAs、snrna、转座元件和基因间区域。许多其他的454序列无法映射到基因组；然而，检查发现了非模板添加和嵌合序列的证据。CPA-sRNAs使用eIF-4E的高亲和力变体捕获5 ' -甲基鸟苷覆盖的RNA，然后进行3 ' -RACE测序。这些结果扩大了丝状真菌中小rna的范围。
Small RNAs are well described in higher eukaryotes such as mammals and plants; however, knowledge in simple eukaryotes such as filamentous fungi is limited. In this study, we discovered and characterized methylguanosine-capped and polyadenylated small RNAs (CPA-sRNAs) by using differential RNA selection, full-length cDNA cloning and 454 transcriptome sequencing of the rice blast fungus Magnaporthe oryzae. This fungus causes blast, a devastating disease on rice, the principle food staple for over half the world’s population. CPA-sRNAs mapped primarily to the transcription initiation and termination sites of protein-coding genes and were positively correlated with gene expression, particularly for highly expressed genes including those encoding ribosomal proteins. Numerous CPA-sRNAs also mapped to rRNAs, tRNAs, snRNAs, transposable elements and intergenic regions. Many other 454 sequence reads could not be mapped to the genome; however, inspection revealed evidence for non-template additions and chimeric sequences. CPA-sRNAs were independently confirmed using a high affinity variant of eIF-4E to capture 5′-methylguanosine-capped RNA followed by 3′-RACE sequencing. These results expand the repertoire of small RNAs in filamentous fungi.
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影响因子：
14.9
作者：
Ebhardt HA;Tsang HH;Dai DC;Liu Y;Bostan B;Fahlman RP
通讯作者：
Fahlman RP
影响因子：
7.4
作者：
Park, Ju-Young;Jin, Jianming;Lee, Yong-Hwan
通讯作者：
Lee, Yong-Hwan
影响因子：
4.5
作者：
Cai, XZ;Hagedorn, CH;Cullen, BR
通讯作者：
Cullen, BR
DOI：
10.1126/science.1181421
发表时间：
2010-01-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nechaev S;Fargo DC;dos Santos G;Liu L;Gao Y;Adelman K
通讯作者：
Adelman K
影响因子：
56.9
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.