High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury.
High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury.
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DOI：
10.3389/fphys.2018.01367
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发表时间：
2018
影响因子：
4
通讯作者：
Rhind SG
中科院分区：
文献类型：
--
作者：
Di Battista AP;Moes KA;Shiu MY;Hutchison MG;Churchill N;Thomas SG;Rhind SG
目的：血液生物标志物是研究脑震荡的有用工具。然而，它们的解释因一些潜在的生物学混淆而变得复杂，包括运动。这在军事和体育环境中尤其相关，因为在这些环境中，损伤通常发生在体力消耗期间。高强度间歇训练(HIIT)对推测的脑损伤生物标志物的影响仍未得到充分研究。本研究的目的是观察HIIT对一组与脑损伤相关的血液生物标志物的影响。方法：11名健康、有运动能力的男性(中位年龄29.0岁，四分位数范围26.0~31.5)在自行车功率机上进行HIIT，间歇时间为8~12×60-S，间歇功率为100%，间歇时间为75-S，功率为50W，每周3次，共2周。在第一次和最后一次训练中，在HIIT之前和之后立即采集外周血样本。用高灵敏度多点免疫分析法检测血浆S100钙结合蛋白β(S100B)、胶质纤维酸性蛋白(GFAP)、神经元特异性烯醇化酶(NSE)、脑源性神经营养因子(BDNF)、神经颗粒素(NRGN)、过氧化还蛋白(PRDX)-6、肌酸激酶BB同工酶(CKBB)、粘蛋白样蛋白(VILIP)-1、血管性血友病因子(VWF)、单核细胞趋化蛋白(MCP)-1、基质金属蛋白酶(MMP)-9和总tau(T-tau)。在重复测量的自举框架内，通过偏最小二乘判别分析(PLSDA)评估对HIIT反应的生物标志物浓度的差异。结果：12个生物标记物中有10个在HIIT前后升高，VILIP-1保持不变，GFAP因检测不足而未进行统计学评估。HIIT后2周，T-tau在HIIT前后不再显著升高，NRGN、PRDX-6、MMP-9和vWF的急性反应明显减弱。此外，与第1组相比，第6组运动前NSE和VILIP-1浓度分别显著降低和升高。结论：通常与脑损伤相关的血液生物标记物在单次HIIT治疗后显著升高。在为期两周、六节课的训练方案后，这一反应对一些标记物有所减弱，但不是所有标记物。虽然生物标记物继续为脑震荡研究提供希望，但未来的研究是必要的，以理清运动和脑损伤的常见生物后遗症。
Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The impact of high-intensity interval training (HIIT) on putative brain injury biomarkers remains under-examined. The purpose of this study was to observe the effects of HIIT on a panel of blood biomarkers associated with brain injury. Methods: Eleven healthy, recreationally active males (median age = 29.0, interquartile range = 26.0–31.5) performed HIIT on a bicycle ergometer (8-12 × 60-s intervals at 100% of peak power output, interspersed by 75-s recovery at 50 W) three times/week for 2 weeks. Peripheral blood samples were collected before and immediately after HIIT during the first and last training sessions. Plasma concentrations of s100 calcium-binding protein beta (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurogranin (NRGN), peroxiredoxin (PRDX)-6, creatine kinase-BB isoenzyme (CKBB), visinin-like protein (VILIP)-1, von Willebrand factor (vWF), monocyte chemoattractant protein (MCP)-1, matrix metalloproteinase (MMP)-9, and total tau (T-tau) were quantitated by high-sensitivity MULTI-SPOT® immunoassay, on the MesoScale Diagnostics electrochemiluminescence detection platform. Differences in biomarker concentrations in response to HIIT were evaluated by partial least squares discriminant analysis (PLSDA) within a repeated-measures bootstrapped framework. Results: Ten of 12 biomarkers were increased pre-to-post HIIT; VILIP-1 remained unchanged, and GFAP was not statistically evaluated due to insufficient detectability. After 2 weeks of HIIT, T-tau was no longer significantly elevated pre-to-post HIIT, and significant attenuation was noted in the acute responses of NRGN, PRDX-6, MMP-9, and vWF. In addition, compared to session 1, session 6 pre-exercise concentrations of NSE and VILIP-1 were significantly lower and higher, respectively. Conclusion: Blood biomarkers commonly associated with brain injury are significantly elevated in response to a single bout of HIIT. After a 2-week, six-session training protocol, this response was attenuated for some, but not all markers. While biomarkers continue to provide promise to concussion research, future studies are necessary to disentangle the common biological sequelae to both exercise and brain injury.
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DOI：
10.1007/s40279-017-0707-1
发表时间：
2017-07
期刊：
Sports medicine (Auckland, N.Z.)
影响因子：
--
作者：
Broglio SP;McCrea M;McAllister T;Harezlak J;Katz B;Hack D;Hainline B;CARE Consortium Investigators
通讯作者：
CARE Consortium Investigators
影响因子：
3.3
作者：
Febbraio, Mark A.
通讯作者：
Febbraio, Mark A.
影响因子：
--
作者：
Bloomer, Richard J.;Fisher-Wellman, Kelsey H.
通讯作者：
Fisher-Wellman, Kelsey H.
DOI：
10.1139/h03-054
发表时间：
2003-10-01
期刊：
CANADIAN JOURNAL OF APPLIED PHYSIOLOGY-REVUE CANADIENNE DE PHYSIOLOGIE APPLIQUEE
影响因子：
--
作者：
Dietrich, MO;Tort, AB;Portela, LV
通讯作者：
Portela, LV
影响因子：
6.9
作者：
Chodobski A;Zink BJ;Szmydynger-Chodobska J
通讯作者：
Szmydynger-Chodobska J