Evaluation of methods for producing renal dysfunction in rats.
Evaluation of methods for producing renal dysfunction in rats.
复制标题
评估大鼠肾功能障碍的方法。
DOI：
10.1002/jps.2600700202
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发表时间：
1981
影响因子：
3.8
通讯作者：
Levy,G
中科院分区：
文献类型：
--
作者：
Giacomini,KM;Roberts,SM;Levy,G
比较了一步5/6肾切除、二步5/6肾切除、双侧输尿管结扎和注射硝酸铀酰等方法对大鼠肾功能的影响。对照组包括单次和两次假手术动物和接受生理盐水注射的动物。根据以下标准评价方法，每天评估6天：存活率、体重、红细胞压积、血清肌酐浓度、血清尿素氮浓度、血清谷氨酸转氨酶活性、血清白蛋白浓度和水杨酸盐的血清蛋白结合率（每隔一天测定）。双侧输尿管结扎的动物仅存活2天，一步5/6肾切除术死亡率很高。一些方法与低白蛋白血症的发生有关，但转氨酶活性没有显著升高。肾功能不全大鼠血清水杨酸蛋白结合率降低。肾功能不全动物血清中肌酐和尿素氮浓度之间呈强正相关血清白蛋白浓度与水杨酸游离分数呈负相关（r= 0.91，P < 0.001）硝酸铀酰注射液具有操作简便、成活率高、安全可靠等优点（本研究中无死亡），以及相对一致和持续的肾功能下降（如血清肌酐和尿素氮浓度所反映）。
The following methods for producing renal dysfunction in rats were compared: single-step 5/6th nephrectomy, two-step 5/6th nephrectomy, bilateral ureteral ligation, and uranyl nitrate injection. Control groups consisted of single and two-step sham-operated animals and animals that received an injection of normal saline solution. The methods were evaluated on the basis of the following criteria, which were assessed daily for 6 days: survival, body weight, hematocrit, serum creatinine concentration, serum urea nitrogen concentration, serum glutamic pyruvic transaminase activity, serum albumin concentration, and serum protein binding of salicylate (determined every other day). Animals with bilateral ureteral ligation survived only 2 days, and single-step 5/6th nephrectomy caused a high incidence of fatalities. Some of the methods were associated with the development of hypoalbuminemia, but no significant elevation of transaminase activity occurred. Serum protein binding of salicylate was reduced in rats with renal dysfunction. A strong positive correlation between the creatinine and urea nitrogen concentrations in the serum of animals with renal dysfunction (r= 0.91,p< 0.001) and a negative correlation between the serum albumin concentration and salicylate free fraction (r= -0.71,p< 0.001) were found. Uranyl nitrate injection has the advantages of technical simplicity, a high survival rate (no deaths in this study), and relatively consistent and sustained diminution of renal function (as reflected by serum creatinine and urea nitrogen concentrations).
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中毒性肾损伤：酶水平的变化。
DOI：
10.1016/0006-2944(77)90051-5
发表时间：
1977
期刊：
Biochemical medicine
影响因子：
--
作者：
E. O. Ngaha;D. Plummer
通讯作者：
D. Plummer
硝酸铀酰诱导的大鼠急性肾衰竭：微穿刺和肾血流动力学研究。
影响因子：
19.6
作者：
W. Flamenbaum;M. L. Huddleston;J. S. McNeil;R. Hamburger
通讯作者：
R. Hamburger
DOI：
--
发表时间：
1944
期刊：
影响因子：
--
作者：
B. Brodie;S. Udenfriend;A. Coburn
通讯作者：
A. Coburn
地高辛肾清除率的决定因素
影响因子：
6.7
作者：
Hillel Halkin;Lewis B. Sheiner;Carl C. Peck;Kenneth L. Melmon
通讯作者：
Kenneth L. Melmon
慢性肾衰竭对大鼠肝脏蛋白质合成和白蛋白代谢的影响。
影响因子：
15.9
作者：
S. Grossman;S. Yap;D. Shafritz
通讯作者：
D. Shafritz