An unexpected RNA distal interaction mode found in an essential region of the hepatitis C virus genome.
An unexpected RNA distal interaction mode found in an essential region of the hepatitis C virus genome.
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DOI：
10.1093/nar/gky074
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发表时间：
2018-05-04
影响因子：
14.9
通讯作者：
Gallego J
中科院分区：
文献类型：
--
作者：
Cantero-Camacho Á;Gallego J
The 3’X tail is a functionally essential 98-nt sequence located at the 3′-end of the hepatitis C virus (HCV) RNA genome. The domain contains two absolutely conserved dimer linkage sequence (DLS) and k nucleotide segments involved in viral RNA dimerization and in a distal base-pairing interaction with stem-loop 5BSL3.2, respectively. We have previously shown that domain 3’X forms an elongated structure comprising two coaxially stacked SL1’ and SL2’ stem-loops. This conformation favors RNA dimerization by exposing a palindromic DLS segment in an apical loop, but buries in the upper stem of hairpin SL2’ the k nucleotides involved in the distal contact with 5BSL3.2. Using nuclear magnetic resonance spectroscopy and gel electrophoresis experiments, here we show that the establishment of the complex between domain 3’X and stem-loop 5BSL3.2 only requires a rearrangement of the nucleotides forming the upper region of subdomain SL2’. The results indicate that the interaction does not occur through a canonical kissing loop mechanism involving the unpaired nucleotides of two terminal loops, but rather involves a base-paired stem and an apical loop and may result in a kissing three-way junction. On the basis of this information we suggest how the 3’X tail switches between monomer, homodimer and heterodimer states to regulate the HCV viral cycle.
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丙型肝炎病毒 RNA 二聚化和核心 RNA 相互作用分析。
影响因子：
14.9
作者：
Ivanyi-Nagy R;Kanevsky I;Gabus C;Lavergne JP;Ficheux D;Penin F;Fossé P;Darlix JL
通讯作者：
Darlix JL
DOI：
10.1093/bioinformatics/btp250
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Darty K;Denise A;Ponty Y
通讯作者：
Ponty Y
影响因子：
5.6
作者：
Chang, KY;Tinoco, I
通讯作者：
Tinoco, I
影响因子：
5.6
作者：
Argaman, L;Altuvia, S
通讯作者：
Altuvia, S
影响因子：
5.4
作者：
Friebe, P;Bartenschlager, R
通讯作者：
Bartenschlager, R