B cells of HIV-1-infected patients bind virions through CD21-complement interactions and transmit infectious virus to activated T cells.
B cells of HIV-1-infected patients bind virions through CD21-complement interactions and transmit infectious virus to activated T cells.
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DOI：
10.1084/jem.192.5.637
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发表时间：
2000-09-04
期刊：
影响因子：
--
通讯作者：
Fauci AS
中科院分区：
文献类型：
--
作者：
Moir S;Malaspina A;Li Y;Chun TW;Lowe T;Adelsberger J;Baseler M;Ehler LA;Liu S;Davey RT Jr;Mican JA;Fauci AS
艾滋病相关免疫发病机制对B细胞的影响在很大程度上与病毒复制的间接后果有关。这项研究表明，艾滋病毒在淋巴组织和外周血中都与B细胞直接相互作用。分别从4名和23名慢性感染患者的淋巴结以及外周血单个核细胞（PBMC）中分离出的B细胞，与来自同一患者的CD4 +细胞相比，在将病毒传递给激活的艾滋病毒阴性PBMC方面表现出相似的能力。然而，与T细胞不同的是，与B细胞相关的病毒是表面结合的，这从其对链霉蛋白酶的敏感性以及艾滋病毒 - 1 RNA原位扩增所显示的染色模式可以看出。细胞分选和配体置换方法确定CD21是B细胞上的艾滋病毒结合受体，并且这种结合是通过补体调理的病毒介导的。还发现这些B细胞与艾滋病毒阴性个体相比，CD21的表达水平显著降低，这表明艾滋病毒对B细胞有直接的干扰作用。这些发现表明，B细胞虽然自身不容易被艾滋病毒感染，但在作为艾滋病毒 - 1的细胞外储存库的能力方面与滤泡树突状细胞相似。此外，B细胞具有在外周血中循环以及在组织中迁移的额外能力，在组织中它们可能与T细胞相互作用并将病毒传递给T细胞。
The impact of HIV-associated immunopathogenesis on B cells has been largely associated with indirect consequences of viral replication. This study demonstrates that HIV interacts directly with B cells in both lymphoid tissues and peripheral blood. B cells isolated from lymph node and peripheral blood mononuclear cells (PBMCs) of 4 and 23 chronically infected patients, respectively, demonstrated similar capacities to pass virus to activated HIV-negative PBMCs when compared with CD4+ cells from the same patients. However, in contrast to T cells, virus associated with B cells was surface bound, as shown by its sensitivity to pronase and the staining pattern revealed by in situ amplification of HIV-1 RNA. Cell sorting and ligand displacing approaches established that CD21 was the HIV-binding receptor on B cells, and that this association was mediated through complement-opsonized virus. These B cells were also found to express significantly lower levels of CD21 compared with HIV-negative individuals, suggesting a direct perturbing effect of HIV on B cells. These findings suggest that B cells, although they themselves are not readily infected by HIV, are similar to follicular dendritic cells in their capacity to serve as extracellular reservoirs for HIV-1. Furthermore, B cells possess the added capability of circulating in peripheral blood and migrating through tissues where they can potentially interact with and pass virus to T cells.
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影响因子：
56.9
作者：
Garside, P;Ingulli, E;Jenkins, MK
通讯作者：
Jenkins, MK
影响因子：
64.8
作者：
EMBRETSON, J;ZUPANCIC, M;HAASE, AT
通讯作者：
HAASE, AT
DOI：
10.1016/0090-1229(92)90066-w
发表时间：
1992-02-01
期刊：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
影响因子：
--
作者：
BENEDETTO, A;DICARO, A;ELIA, G
通讯作者：
ELIA, G
影响因子：
56.9
作者：
BERBERIAN, L;GOODGLICK, L;BRAUN, J
通讯作者：
BRAUN, J
影响因子：
64.8
作者：
MELCHERS, F;ERDEI, A;DIERICH, MP
通讯作者：
DIERICH, MP