DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria.
DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria.
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DEAD-Box RNA 解旋酶 DDX47 维持飞蝗中肠稳态
DOI：
10.3390/ijms23020586
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发表时间：
2022-01-06
影响因子：
5.6
通讯作者：
Xing SP
中科院分区：
文献类型：
--
作者：
Wang JX;Ma EB;Zhang JZ;Xing SP
组织动态平衡对于维持器官的形状、大小和功能至关重要。这种状态是由新细胞生成和衰老细胞丢失之间的平衡来调节的，涉及到许多因素和机制。中肠是肠道的重要组成部分，负责昆虫的消化和营养吸收。LmDDX47是蝗虫Dead-box helicase 47的同源基因，对于维持若虫正常的中肠是必不可少的。然而，其潜在的细胞和分子机制仍有待阐明。在本研究中，LmDDX47基因敲除导致若虫和成虫的中肠和胃盲肠萎缩。LmDDX47基因敲除后，中肠再生细胞和柱状细胞数量明显减少，柱状组织细胞死亡。LmDDX47定位于核仁，这与LmDDX47基因敲除组18S rRNA合成减少一致。此外，中肠蛋白的乙酰化和巴豆化水平显著提高。因此，LmDDX47可能是蝗虫中肠动态平衡的关键调节因子，调节18S rRNA的合成以及蛋白质的乙酰化和巴豆化。
Tissue homeostasis is critical for maintaining organ shape, size, and function. The condition is regulated by the balance between the generation of new cells and the loss of senescent cells, and it involves many factors and mechanisms. The midgut, an important part of the intestinal tract, is responsible for digestion and nutrient absorption in insects. LmDDX47, the ortholog of DEAD-box helicase 47 from Locusta migratoria, is indispensable for sustaining a normal midgut in the nymphs. However, the underlying cellular and molecular mechanisms remain to be elucidated. In this study, LmDDX47 knockdown resulted in atrophy of the midgut and gastric cecum in both nymph and adult locusts. After LmDDX47 knockdown, the number of regenerative and columnar cells in the midgut was significantly reduced, and cell death was induced in columnar tissue. LmDDX47 was localized to the nucleolus; this was consistent with the reduction in 18S rRNA synthesis in the LmDDX47 knockdown group. In addition, the acetylation and crotonylation levels of midgut proteins were significantly increased. Therefore, LmDDX47 could be a key regulator of midgut homeostasis, regulating 18S rRNA synthesis as well as protein acetylation and crotonylation in the migratory locust.
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影响因子：
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