G protein γ subunit qPE9-1 is involved in rice adaptation under elevated CO(2) concentration by regulating leaf photosynthesis.
G protein γ subunit qPE9-1 is involved in rice adaptation under elevated CO(2) concentration by regulating leaf photosynthesis.
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G蛋白γ亚基qPE9-1通过调节叶片光合作用参与水稻在CO2浓度升高下的适应
DOI：
10.1186/s12284-021-00507-7
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发表时间：
2021-07-15
期刊：
影响因子：
--
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Wang K;Xu F;Yuan W;Sun L;Wang S;Aslam MM;Zhang J;Xu W
G蛋白γ亚基qPE9 - 1在水稻生长发育中起着多重作用。然而，qPE 9 - 1在暴露于升高的二氧化碳浓度（eCO2）的水稻中的作用尚不清楚。在此，我们使用qPE9 - 1过表达（OE）系、RNAi系和相应的WT水稻研究了其在eCO2条件下对水稻生长的调节作用。与大气二氧化碳浓度（aCO2）相比，eCO2胁迫下水稻叶片qPE9 - 1的相对表达量约高10倍。在eCO2下，WT和qPE9 - 1过表达水稻的生长显著高于aCO2下。此外，eCO2对qPE9 - 1 RNAi株系的生长没有显著影响。此外，野生型和qPE9 - 1过表达水稻表现出更高的净光合速率和碳水化合物含量下eCO2比下aCO2。此外，在eCO2下，一些光合作用相关基因在WT中的相对表达量在RNA-seq分析中显示出显著差异，而在RNAi 3系中没有。在eCO2胁迫下，rbcL基因在qPE9 - 1过表达水稻叶片中的表达量和Rubisco含量均高于野生型和RNAi水稻。这些结果表明，qPE9 - 1通过调节水稻光合光反应和Rubisco含量来调节叶片光合作用，从而参与水稻对CO2浓度升高的适应。在线版本包含补充材料，可通过10.1186/s12284 - 021 - 00507 - 7获得。
G protein γ subunit qPE9-1 plays multiple roles in rice growth and development. However, the role of qPE9-1 in rice exposed to elevated carbon dioxide concentration (eCO2) is unknown. Here, we investigated its role in the regulation of rice growth under eCO2 conditions using qPE9-1 overexpression (OE) lines, RNAi lines and corresponding WT rice. Compared to atmospheric carbon dioxide concentration (aCO2), relative expression of qPE9-1 in rice leaf was approximately tenfold higher under eCO2. Under eCO2, the growth of WT and qPE9-1-overexpressing rice was significantly higher than under aCO2. Moreover, there was no significant effect of eCO2 on the growth of qPE9-1 RNAi lines. Furthermore, WT and qPE9-1-overexpressing rice showed higher net photosynthetic rate and carbohydrate content under eCO2 than under aCO2. Moreover, the relative expression of some photosynthesis related genes in WT, but not in RNAi3 line, showed significant difference under eCO2 in RNA-seq analysis. Compared to WT and RNAi lines, the rbcL gene expression and Rubisco content of rice leaves in qPE9-1-overexpressors were higher under eCO2. Overall, these results suggest that qPE9-1 is involved in rice adaptation under elevated CO2 concentration by regulating leaf photosynthesis via moderating rice photosynthetic light reaction and Rubisco content. The online version contains supplementary material available at 10.1186/s12284-021-00507-7.
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13.8
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