Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit.
Pseudomonas bijieensis Strain XL17 within the P. corrugata Subgroup Producing 2,4-Diacetylphloroglucinol and Lipopeptides Controls Bacterial Canker and Gray Mold Pathogens of Kiwifruit.
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产生2，4-二乙酰间苯三酚和脂肽的波纹假单胞菌亚群中的假单胞菌XL17能控制猕猴桃溃烂病和灰霉病。
DOI：
10.3390/microorganisms10020425
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发表时间：
2022-02-12
期刊：
影响因子：
4.5
通讯作者：
An Q
中科院分区：
文献类型：
--
作者：
Ali MA;Luo J;Ahmed T;Zhang J;Xie T;Dai D;Jiang J;Zhu J;Hassan S;Alorabi JA;Li B;An Q
猕猴桃细菌性溃疡病是世界范围内猕猴桃的主要病害之一。放线菌病（Psa）和灰霉病（Botrytis cinerea）引起的灰霉病。本研究从油菜根癌中分离到一株内生细菌XL 17，该细菌对Psa和B具有较强的拮抗活性。灰色区域。菌株XL 17及其无细胞培养滤液（CF）对Psa和B的生长均有抑制作用。灰霉病、Psa相关的叶坏死和B.猕猴桃坏死病电镜观察发现XL 17 CF可损伤Psa和B细胞的结构。灰色区域。基于基因组的分类表明，菌株XL 17属于荧光假单胞菌种复合体的皱皮假单胞菌亚组中的毕节假单胞菌。在包含31个基因组种的皱叶侧耳亚组中，phl操纵子的存在和脂肽/群体感应岛的缺失可以作为决定植物保护生活方式的遗传标记，其中phl操纵子负责酚聚酮2，4-二乙酰间苯三酚（DAPG）的生物合成。HPLC检测到XL 17 CF浸提液中的DAPG。MALDI-TOF-MS分析表明菌株XL 17产生粘蛋白家族和orfamide家族的环脂肽。总之，表型、基因组和代谢分析鉴定了产生DAPG和环脂肽的毕节假单胞菌XL 17可用于控制猕猴桃的细菌性溃疡病和灰霉病病原体。
Kiwifruit worldwide suffers from the devastating diseases of bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) and gray mold caused by Botrytis cinerea. Here, an endophytic bacterium XL17 isolated from a rape crown gall was screened out for its potent antagonistic activities against Psa and B. cinerea. Strain XL17 and its cell-free culture filtrate (CF) inhibited the growth of Psa and B. cinerea, Psa-associated leaf necrosis, and B. cinerea-associated kiwifruit necrosis. Electron microscopy showed that XL17 CF could damage the cell structures of Psa and B. cinerea. Genome-based taxonomy revealed that strain XL17 belongs to Pseudomonas bijieensis within the P. corrugata subgroup of the P. fluorescens species complex. Among the P. corrugata subgroup containing 31 genomospecies, the presence of the phl operon responsible for the biosynthesis of the phenolic polyketide 2,4-diacetylphloroglucinol (DAPG) and the absence of the lipopeptide/quorum sensing island can serve as the genetic marker for the determination of a plant-protection life style. HPLC detected DAPG in extracts from XL17 CF. MALDI-TOF-MS analysis revealed that strain XL17 produced cyclic lipopeptides of the viscosin family and orfamide family. Together, phenotypic, genomic, and metabolic analyses identified that P. bijieensis XL17 producing DAPG and cyclic lipopeptides can be used to control bacterial canker and gray mold pathogens of kiwifruit.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4.5
作者：
Girard L;Lood C;Höfte M;Vandamme P;Rokni-Zadeh H;van Noort V;Lavigne R;De Mot R
通讯作者：
De Mot R
影响因子：
3.7
作者：
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通讯作者：
Li, Bin
影响因子：
5.2
作者：
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通讯作者：
Muller, Daniel
影响因子：
1.7
作者：
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通讯作者：
Pevzner, Pavel A.