Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation.
Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation.
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DOI：
10.1016/j.physbeh.2009.04.010
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发表时间：
2009-08-04
影响因子：
2.9
通讯作者：
Pavlides, Constantine
中科院分区：
文献类型：
--
作者：
Romcy-Pereira, Rodrigo N.;Erraji-Benchekroun, Loubna;Smyrniotopoulos, Peggy;Ogawa, Sonoko;Mello, Claudio V.;Sibille, Etienne;Pavlides, Constantine
关键词：
The activity-dependent transcription factor zif268 is re-activated in sleep following hippocampal long-term potentiation (LTP). However, the activation of secondary genes, possibly involved in modifying local synaptic strengths and ultimately stabilizing memory traces during sleep, has not yet been studied. Here, we investigated changes in hippocampal and cortical gene expression at a time point subsequent to the previously reported initial zif268 re-activation during sleep. Rats underwent unilateral hippocampal LTP and were assigned to SLEEP or AWAKE groups. Eighty minutes after a long rapid-eye-movement sleep (REMS) episode (or an equivalent amount of time for awake group) animals had their hippocampi dissected and processed for gene microarray hybridization. Prefrontal and parietal cortices were also collected for qRT-PCR analysis. The microarray analysis identified 28 up-regulated genes in the hippocampus: 11 genes were enhanced in the LTPed hemisphere of sleep animals; 13 genes were enhanced after sleep, regardless of hemisphere; and 4 genes were enhanced in LTPed hemisphere, regardless of behavioral state. qRT-PCR analysis confirmed the upregulation of aif-1 and sc-65 during sleep. Moreover, we observed a down-regulation of the purinergic receptor, P2Y4R in the LTP hemisphere of awake animals and a trend for the protein kinase, CaMKI to be up-regulated in the LTP hemisphere of sleep animals. In the prefrontal cortex, we showed a significant LTP-dependent down-regulation of gluR1 and spinophilin specifically during sleep. Zif268 was downregulated in sleep regardless of the hemisphere. No changes in gene expression were observed in the parietal cortex. Our findings indicate that a set of synaptic plasticity-related genes have their expression modulated during sleep following LTP, which can reflect biochemical events associated with reshaping of synaptic connections in sleep following learning.
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影响因子：
5.3
作者：
Li, L;Carter, J;Tourtellotte, WG
通讯作者：
Tourtellotte, WG
影响因子：
16.2
作者：
Bozon, B;Davis, S;Laroche, S
通讯作者：
Laroche, S
影响因子：
3.4
作者：
Herry, C;Mons, N
通讯作者：
Mons, N
影响因子：
5.3
作者：
Bauer, EP;Schafe, GE;LeDoux, JE
通讯作者：
LeDoux, JE
DOI：
10.1139/o92-147
发表时间：
1992-10-01
期刊：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
影响因子：
--
作者：
CHEN, QF;PEARLMAN, RE;MOENS, PB
通讯作者：
MOENS, PB