NBCe1 (SLC4A4) a potential pH regulator in enamel organ cells during enamel development in the mouse.
NBCe1 (SLC4A4) a potential pH regulator in enamel organ cells during enamel development in the mouse.
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DOI：
10.1007/s00441-014-1935-4
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发表时间：
2014-11
影响因子：
3.6
通讯作者：
Bronckers, A. L. J. J.
中科院分区：
文献类型：
--
作者：
Jalali, R.;Guo, J.;Zandieh-Doulabi, B.;Bervoets, T. J. M.;Paine, M. L.;Boron, W. F.;Parker, M. D.;Bijvelds, M. J. C.;Medina, J. F.;DenBesten, P. K.;Bronckers, A. L. J. J.
关键词：
在牙釉质形成的成熟阶段，成釉细胞表达离子转运跨膜蛋白。SLC 4家族的离子转运蛋白通过共同转运HCO 3 −和Na+来调节真核细胞内和细胞外的pH值。SLC 4A 4（编码Na+碳酸氢盐协同转运蛋白NBCe 1）突变可诱导人类和小鼠牙釉质发育缺陷。我们假设，NBCe 1在牙齿上皮细胞从事中和质子在釉质空间中的晶体形成过程中释放。我们免疫定位了小鼠野生型牙上皮中的NBCe 1蛋白，并检查了NBCe 1缺失突变对小鼠牙釉质形成的影响。成釉细胞表达NBCe 1亚型B/D/C/E的基因转录本。在野生型小鼠中，用识别亚型A/B/D/E和亚型C的抗体在分泌期的成釉细胞中观察到弱至中度的NBCe 1免疫染色，在早期成熟阶段没有或非常低的染色，但在晚期成熟阶段中至高的染色。乳头层则相反，在成熟早期染色较强，而在成熟中期和晚期染色逐渐减弱。在NBCe 1 −/−小鼠中，成釉细胞紊乱，釉质薄且严重矿化不足。CFTR−/−和AE 2a，B−/−小鼠（被认为是成釉细胞中的pH调节剂）的釉质器官比野生型小鼠含有更高水平的NBCe 1蛋白。我们的数据表明，NBCe 1在成釉细胞和乳头层细胞的表达依赖于发育阶段，并可能响应pH值的变化。
During formation of dental enamel maturation-stage ameloblasts express ion-transporting transmembrane proteins. The SLC4 family of ion-transporters regulates intra- and extracellular pH in eukaryotic cells by co-transporting HCO3− with Na+. Mutation in SLC4A4 (coding for the Na+ bicarbonate co-transporter NBCe1) induces developmental defects in human and murine enamel. We hypothesized that NBCe1 in dental epithelium is engaged in neutralizing protons released during crystal formation in the enamel space. We immunolocalized NBCe1 protein in mouse wild-type dental epithelium and examined the effect of NBCe1-null mutation on enamel formation in mice. Ameloblasts expressed gene transcripts for NBCe1 isoforms B/D/C/E. In wild-type mice weak to moderate immunostaining for NBCe1 with antibodies that recognize isoforms A/B/D/E and isoform C was seen in ameloblasts in secretory stage, no or very low staining in early maturation-stage but moderately to high staining in late maturation-stage. The papillary layer showed the opposite pattern and immunostained prominently at early maturation-stage but gradually showed less staining at mid- and late maturation-stage. In NBCe1−/− mice ameloblasts were disorganized, the enamel thin and severely hypomineralized. Enamel organs of CFTR−/− and AE2a,b−/− mice (believed to be pH regulators in ameloblasts) contained higher levels of NBCe1 protein than wild-type mice. Our data show that expression of NBCe1 in ameloblast and papillary layer cell depends on developmental stage and possibly responds to pH changes.
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影响因子：
7.6
作者：
Lyaruu, D. M.;Medina, J. F.;Bronckers, A. L. J. J.
通讯作者：
Bronckers, A. L. J. J.
影响因子：
5.6
作者：
Lacruz, Rodrigo S.;Smith, Charles E.;Moffatt, Pierre;Chang, Eugene H.;Bromage, Timothy G.;Bringas, Pablo, Jr.;Nanci, Antonio;Baniwal, Sanjeev K.;Zabner, Joseph;Welsh, Michael J.;Kurtz, Ira;Paine, Michael L.
通讯作者：
Paine, Michael L.
影响因子：
4.2
作者：
Lacruz, Rodrigo S.;Nanci, Antonio;Kurtz, Ira;Wright, J. Timothy;Paine, Michael L.
通讯作者：
Paine, Michael L.
DOI：
10.1152/ajprenal.00612.2012
发表时间：
2013-04-01
影响因子：
4.2
作者：
Lee, Seong-Ki;Boron, Walter F.;Parker, Mark D.
通讯作者：
Parker, Mark D.
影响因子：
13.5
作者：
Uriarte, Iker;Banales, Jesus M.;Medina, Juan F.
通讯作者：
Medina, Juan F.