Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries).
Metabolic Effects of FecB Gene on Follicular Fluid and Ovarian Vein Serum in Sheep (Ovis aries).
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FecB基因对绵羊卵泡液和卵巢静脉血清代谢的影响
DOI：
10.3390/ijms19020539
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发表时间：
2018-02-11
影响因子：
5.6
通讯作者：
Chu M
中科院分区：
文献类型：
--
作者：
Guo X;Wang X;Di R;Liu Q;Hu W;He X;Yu J;Zhang X;Zhang J;Broniowska K;Chen W;Wu C;Chu M
FECB基因是绵羊的一个重要基因，与排卵率密切相关，但其调控机制尚不清楚。本研究采用液质联用(LC-MS)和气质联用(GC-MS)技术对小尾寒羊卵泡液和卵巢静脉血清中FECB基因的代谢效应进行了检测。采用方差分析和随机森林统计方法确定重要的代谢途径和生物标志物。观察了三种FECB基因STH母羊在FF中氨基酸代谢、氧化还原环境和能量代谢的变化。主成分分析(PCA)和系统聚类分析(HCA)表明，FECB基因的代谢效应在卵母细胞中比在卵泡中更明显。因此，FECB基因型别也会影响FECB代谢谱的差异。在Spearman相关分析中，绵羊FF样品中的关键代谢物(如葡萄糖6-磷酸、葡萄糖1-磷酸、天冬氨酸、天冬酰胺、氧化谷胱甘肽、半胱氨酸-谷胱甘肽二硫化物、γ-谷氨酰胺和2-羟丁酸)与排卵率显著相关。我们的研究结果将有助于解释高繁殖力母羊的代谢机制，有利于繁殖力鉴定。
The FecB gene has been discovered as an important gene in sheep for its high relationship with the ovulation rate, but its regulatory mechanism remains unknown. In the present study, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) techniques were adopted to detect the metabolic effects of FecB gene in follicular fluid (FF) and ovarian vein serum (OVS) in Small Tail Han (STH) sheep. ANOVA and random forest statistical methods were employed for the identification of important metabolic pathways and biomarkers. Changes in amino acid metabolism, redox environment, and energy metabolism were observed in FF from the three FecB genotype STH ewes. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) showed that metabolic effects of FecB gene are more pronounced in FF than in OVS. Therefore, the difference of the metabolic profile in FF is also affected by the FecB genotypes. In Spearman correlation analysis, key metabolites (e.g., glucose 6-phosphate, glucose 1-phosphate, aspartate, asparagine, glutathione oxidized (GSSG), cysteine-glutathione disulfide, γ-glutamylglutamine, and 2-hydrosybutyrate) in ovine FF samples showed a significant correlation with the ovulation rate. Our findings will help to explain the metabolic mechanism of high prolificacy ewes and benefit fertility identification.
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影响因子：
3.8
作者：
Campbell, BK;Baird, DT;Webb, R
通讯作者：
Webb, R
影响因子：
4.8
作者：
D'Aniello, A;Di Fiore, MM;Ingrosso, D
通讯作者：
Ingrosso, D
影响因子：
6.7
作者：
Dumesic, Daniel A.;Meldrum, David R.;Schoolcraft, William B.
通讯作者：
Schoolcraft, William B.
影响因子：
6.1
作者：
D'Aniello, G.;Grieco, N.;Ronsini, S.
通讯作者：
Ronsini, S.
影响因子：
3.6
作者：
Hanrahan, JP;Gregan, SM;Galloway, SM
通讯作者：
Galloway, SM