Highly Sensitive Two-Dimensional Paper Network Incorporating Biotin-Streptavidin for the Detection of Malaria.
Highly Sensitive Two-Dimensional Paper Network Incorporating Biotin-Streptavidin for the Detection of Malaria.
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DOI：
10.1021/acs.analchem.5b03999
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发表时间：
2016-03-01
影响因子：
7.4
通讯作者：
Richards-Kortum R
中科院分区：
文献类型：
--
作者：
Grant BD;Smith CA;Karvonen K;Richards-Kortum R
最近，已经开发了二维纸网络，以使多步测定能够以侧向流格式进行。这些装置已被用于在纸上进行简单的酶联免疫测定。然而，这些设备尚未纳入更复杂的免疫测定，包括使用链霉亲和素-生物素检测策略。在这里，我们提出了一个修改后的二维纸网络能够连续提供六种试剂。该装置仅需要单个用户步骤，并递送（i）样品，（ii）生物素化的检测抗体，（iii）链霉亲和素辣根过氧化物酶，（iv）洗涤缓冲液，（v）比色底物和（vi）最终洗涤缓冲液。为了证明这种方法的实用性，我们设计了一种检测疟疾蛋白Pf HRP 2的方法。使用该平台，我们能够实现与传统96孔板夹心ELISA相当的检测限。除了检测限的改进外，链霉亲和素-生物素的加入简化了其他靶标的类似检测的开发。
Recently, two-dimensional paper networks have been developed to enable multistep assays to be performed in a lateral flow format. These devices have been used to perform simple enzyme linked immunoassays on paper. However, these devices have yet to incorporate more complex immunoassays, including the use of streptavidin-biotin detection strategies. Here we present a modified two-dimensional paper network capable of consecutively delivering six reagents. The device requires only a single user step and delivers (i) the sample, (ii) the biotinylated detection antibody, (iii) streptavidin horse-radish peroxidase (iv) a wash buffer (v) a colorimetric substrate and (vi) a final wash buffer. To demonstrate the utility of this approach we designed an assay to detect the malaria protein Pf HRP2. Using this platform, we were able to achieve a limit-of-detection equivalent to that of a traditional 96-well plate sandwich ELISA. In addition to improvements in the limit-of-detection, the inclusion of streptavidin-biotin simplifies the development of similar tests for other targets.
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影响因子：
168.9
作者：
BEADLE, C;LONG, GW;HOFFMAN, SL
通讯作者：
HOFFMAN, SL
影响因子：
3
作者：
Butterworth AS;Robertson AJ;Ho MF;Gatton ML;McCarthy JS;Trenholme KR
通讯作者：
Trenholme KR
影响因子：
8.4
作者：
Fu, Elain;Kauffman, Peter;Lutz, Barry;Yager, Paul
通讯作者：
Yager, Paul
影响因子：
7.4
作者：
Fridley, Gina E.;Le, Huy;Yager, Paul
通讯作者：
Yager, Paul
影响因子：
7.4
作者：
Martinez, Andres W.;Phillips, Scott T.;Carrilho, Emanuel
通讯作者：
Carrilho, Emanuel