Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139
Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139
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面包小麦中源自二粒小麦的条锈病抗性基因 YrSM139-1B 的分子作图和标记开发
DOI：
10.1007/s00122-015-2633-7
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发表时间：
2016-02
影响因子：
5.4
通讯作者：
Ji WQ
中科院分区：
文献类型：
--
作者：
Zhang H.;Zhang L.;Wang CY;Wang YJ;Zhou XL;Lv SK;Liu XL;Kang ZS;Ji WQ
YrSM 139 -1B可能是一个新的有效抗条锈病基因，并开发了可用于分子标记辅助选择的侧翼标记。小麦赤霉病是小麦的一种重要叶部病害。小麦品种陕麦139的抗条锈基因YrSM 139 -1B和YrSM 139 -2D分别来自野生二粒小麦和中间偃麦草。以陕麦139 ×沪901-19为材料，通过分子标记辅助选择，获得了3对近等基因F7：8（对照重组自交系）：N122- 1013 R/S、N122 - 185 R/S和N122- 1812 R/S。利用90 K Illumina iSelect SNP阵列开发的SSR表达序列标签（EST）和位点特异性序列标记，对N122 - 1013 R × N122- 1013 S的F2：3群体进行条锈病反应检测，并对1BS 10 - 0. 5 bin区域的标记进行分析。5个EST-STS标记和4个等位基因特异性PCR标记被定位到YrSM 139 -1B区域。YrSM 139 -1B的30.5cM遗传图谱由9个标记组成，其中两个标记与YrSM 139 -1B的距离比Xgwm 273近，Xgwm 273用于产生对照RIL对。小种反应数据和等位性测试表明，YrSM 139 -1B不同于Yr 10、Yr 15和Yr 24/26/CH 42。
Key message:YrSM139-1B maybe a new gene for effective resistance to stripe rust and useful flanking markers for marker-assisted selection were developed.Abstract:Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important foliar disease of wheat. Two dominant stripe rust resistant genes YrSM139-1B and YrSM139-2D were pyramided in bread wheat cultivar Shaanmai 139; one from wild emmer and the other from Thinopyrum intermedium. Three near-isogenic F7:8 line pairs (contrasting RILs), N122-1013R/S, N122-185R/S, and N122-1812R/S, independently derived from different F2 plants and differing at the YrSM139-1B locus were generated from the cross Shaanmai 139 × Hu 901-19 through marker-assisted selection. A large F2:3 population from cross N122-1013R × N122-1013S tested for stripe rust response and subjected to analysis with markers in the 1BS10-0.5 bin region using SSR expressed sequence tags (EST) and site-specific sequence markers developed from the 90 K Illumina iSelect SNP array. Five EST-STS markers and four allele-specific PCR markers were mapped to the YrSM139-1B region. The 30.5 cM genetic map for YrSM139-1B consisted of nine markers, two of which were closer to YrSM139-1B than Xgwm273, which was used in producing the contrasting RIL pairs. Race response data and allelism tests showed that YrSM139-1B is different from Yr10, Yr15, and Yr24/26/CH42.
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DOI：
10.3724/sp.j.1006.2010.00109
发表时间：
2010-02
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Z. Hong;Ren Zhilong;Hu Yingang;Wang Changyou;Jiang Wanquan
通讯作者：
Z. Hong;Ren Zhilong;Hu Yingang;Wang Changyou;Jiang Wanquan
中国小麦地方品种葫芦头白粉病抗性基因MlHLT的遗传与物理定位
DOI：
10.1007/s00122-014-2436-2
发表时间：
2015-02
期刊：
Theor Appl Genet
影响因子：
--
作者：
Wang Zhenzhong, Hanwen Li, Deyun Zhang, Li Guo, Jiaojiao Chen
通讯作者：
Wang Zhenzhong, Hanwen Li, Deyun Zhang, Li Guo, Jiaojiao Chen
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Zhao Jie
通讯作者：
Zhao Jie
影响因子：
2
作者：
R. McIntosh;E. Lagudah
通讯作者：
R. McIntosh;E. Lagudah
影响因子：
4.8
作者：
Yong-pan Ren;Sheng-rong Li;Yuming Wei;Qiang Zhou;Xiao-ying Du;Yuanjiang He;Youliang Zheng
通讯作者：
Yong-pan Ren;Sheng-rong Li;Yuming Wei;Qiang Zhou;Xiao-ying Du;Yuanjiang He;Youliang Zheng