Comparison of humoral immune responses and tumor immunity in mice immunized with recombinant SV40 large tumor antigen and a monoclonal anti-idiotype.
Comparison of humoral immune responses and tumor immunity in mice immunized with recombinant SV40 large tumor antigen and a monoclonal anti-idiotype.
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用重组SV40大肿瘤抗原和单克隆抗独特型免疫小鼠的体液免疫反应和肿瘤免疫的比较。
作者：
M. Shearer;R. K. Bright;R. Kennedy
我们比较了重组SV40大肿瘤抗原（T-ag）免疫在BALB/c小鼠中诱导的体液免疫应答与SV40 T-ag诱导的Id网络组分特异性的单克隆抗独特型（anti-Id） 58D诱导的体液免疫应答。我们还用致死剂量的sv40转化细胞刺激免疫小鼠，以评估体内肿瘤免疫。两周接种两次SV40 T-ag或抗id 58D诱导的体液反应同时识别SV40 T-ag和抗id 58D。每两周接种四次SV40 T-ag可提高抗原特异性抗体滴度，降低对抗id 58D的反应，而每两周接种四次抗id 58D可提高对自身和SV40 T-ag的抗体滴度。特异性T-ag表位和独特位特异性的比较表明，SV40 T-ag和抗id 58D免疫产生的应答识别SV40 T-ag上相似的表位，并表达抗id 58D识别的共享独特位。用致死剂量的SV40转化细胞攻击SV40 T-ag免疫小鼠，完全受到保护，未观察到肿瘤。尽管事实上很少或没有检测到SV40 t -ag特异性细胞毒性t淋巴细胞活性。相比之下，10只免疫了anti-Id 58D的小鼠中只有3只免受致命攻击。这些结果表明，尽管单克隆抗- id免疫可以诱导识别相似的SV40 T-ag表位并表达与SV40 T-ag抗体相关的共享独特位的应答，但重组抗原本身可诱导更好的体内肿瘤免疫。
We compared the humoral immune responses induced in BALB/c mice by immunization with recombinant SV40 large tumor antigen (T-ag) with those induced by a monoclonal anti-idiotype (anti-Id), designated 58D, that is specific for SV40 T-ag-induced Id network components. We also challenged immunized mice with a lethal dose of SV40-transformed cells to assess in vivo tumor immunity. Two biweekly immunization with either SV40 T-ag or anti-Id 58D induced humoral responses that recognized both SV40 T-ag and anti-Id 58D. Four biweekly immunizations with SV40 T-ag increased the antigen-specific antibody titers and decreased the response to anti-Id 58D, while four biweekly immunizations of anti-Id 58D increased antibody titers to both itself and SV40 T-ag. Comparison of specific T-ag epitope and idiotope specificities indicated that SV40 T-ag and anti-Id 58D immunization generated responses that recognized a similar epitope on SV40 T-ag and expressed a shared idiotope recognized by anti-Id 58D. SV40 T-ag immunized mice challenged with a lethal dose of SV40-transformed cells were completely protected and no tumors were observed. This is despite the fact that little or no SV40 T-ag-specific cytotoxic T-lymphocyte activity was detectable. In contrast, only 3 of 10 mice immunized with anti-Id 58D were protected from a lethal challenge. These results indicate that, although monoclonal anti-Id immunization can induce responses that recognize similar SV40 T-ag epitopes and express shared idiotopes associated with antibodies to SV40 T-ag, the recombinant antigen itself induces superior in vivo tumor immunity.
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影响因子：
8
作者：
J. Yokota;T. Akiyama;Y. Fung;W. Benedict;Y. Namba;M. Hanaoka;M. Wada;T. Terasaki;Y. Shimosata;Takashi Sugimura;M. Terada
通讯作者：
J. Yokota;T. Akiyama;Y. Fung;W. Benedict;Y. Namba;M. Hanaoka;M. Wada;T. Terasaki;Y. Shimosata;Takashi Sugimura;M. Terada
使用抗独特型抗体的主动免疫策略。
DOI：
10.1016/b978-0-7506-9265-6.50023-3
发表时间：
1992
期刊：
Biotechnology (Reading, Mass.)
影响因子：
--
作者：
Mernaugh,RL;Bright,RK;Kennedy,RC
通讯作者：
Kennedy,RC
鼠单克隆抗独特型抗体作为人类癌胚抗原的潜在网络抗原。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bhattacharya-Chatterjee,M;Mukerjee,S;Biddle,W;Foon,KA;Kohler,H
通讯作者：
Kohler,H
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Raychaudhuri,S;Kang,CY;Kaveri,SV;Kieber-Emmons,T;Kohler,H
通讯作者：
Kohler,H
DOI：
10.1172/jci113195
发表时间：
1987-11
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. Kennedy;E. Zhou;R. Lanford;T. Chanh;C. Bona
通讯作者：
R. Kennedy;E. Zhou;R. Lanford;T. Chanh;C. Bona