Tyrosine Kinase-Mediated Activation of Cytosolic Calcium Oscillations and Phastic Myometrical Contractions
Tyrosine Kinase-Mediated Activation of Cytosolic Calcium Oscillations and Phastic Myometrical Contractions
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酪氨酸激酶介导的胞质钙振荡和肌力收缩的激活
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Phillippe
中科院分区：
文献类型：
--
作者：
J. Ascher‐Landsberg;T. Saunders;M. Phillippe
这些研究试图检验酪氨酸激酶刺激的阶段性肌收缩是由磷脂酰肌醇（PI）信号通路的激活和胞质钙振荡的产生介导的假设。对于这些研究，在周期的发情前期/发情期从成年雌性Sprague-Dawley白色大鼠中获得子宫组织。使用过钒酸盐（一种酪氨酸磷酸酶抑制剂）进行体内收缩研究，同时使用和不使用PI信号通路抑制剂，包括2-硝基-4-羧基苯基-N，N-二苯基氨基甲酸酯（一种磷脂酶C抑制剂）、硫柳汞（一种肌醇-三磷酸受体/通道抑制剂）和钌红（一种兰尼碱受体抑制剂），以及催产素或前列腺素F2α（两种经典的子宫收缩激动剂）。使用Fura-2负载的子宫肌条进行细胞溶质钙研究。在这些研究中，观察到过钒酸盐在子宫肌组织中产生细胞质钙振荡和相位收缩，与催产素和前列腺素F2α产生的反应相当。在抑制磷脂酶C，肌醇-三磷酸受体，和ryanodine受体，从而证实了PI信号通路的重要性，在酪氨酸激酶相关的肌电活动的pervandate刺激的阶段性收缩显着抑制。进一步证实了PI信号通路在过钒酸盐刺激的肌收缩中的重要和共同作用，当经典的子宫收缩激动剂如催产素或前列腺素F2α与过钒酸盐联合使用时，未观察到显著的累加效应。
These studies sought to test the hypothesis that tyrosine kinase-stimulated phasic myometrical contractions are mediated by activation of the phosphatidylinositol (PI)-signaling pathway and the generation of cytosolic calcium oscillations. For these studies, uterine tissue was obtained from adult female Sprague-Dawley white rats during the proestrus/estrus phase of the cycle. In vivo contraction studies were performed using pervanadate (a tyrosine phosphatase inhibitor) with and without inhibitors of the PI-signaling pathway, including 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (a phospholipase C inhibitor), thimerosal (an inositol-trisphosphate receptor/channel inhibitor), and Ruthenium red (a ryanodine receptor inhibitor), and with oxytocin or prostaglandin F2α (two classic uterotonic agonists). Cytosolic calcium studies were performed using Fura-2-loaded myometrial strips. During these studies, pervanadate was observed to produce cytosolid calcium oscillations and phasic contractions in myometrical tissue comparable to those produced in response to oxytocin and prostaglandin F2α. The pervandate-stimulated phasic contractions were significantly suppressed in response to inhibition of phospholipase C, the inositol-trisphosphate receptor, and the ryanodine receptor, thereby confirming the importance of the PI-signaling pathway during tyrosine kinase-associated myometrical activity. Further confirming the important and shared role for the PI-signaling pathway during pervanadate-stimulated myometrical contractions, no significant additive effects were observed when classic uterotonic agonists such as oxytocin or prostaglandin F2α were combined with pervanadate.
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影响因子：
9.8
作者：
Phillippe,M
通讯作者：
Phillippe,M
影响因子：
4.8
作者：
C. Ku;Ansha Qian;Yeshao Wen;Khursheed Anwer;Barbara M. Sanborn
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DOI：
10.1006/bbrc.1997.6163
发表时间：
1997
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Phillippe,M;Basa,A
通讯作者：
Basa,A
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DOI：
10.1006/bbrc.1995.1769
发表时间：
1995
期刊：
Biochemical and biophysical research communications.
影响因子：
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作者：
Phillippe,M
通讯作者：
Phillippe,M
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影响因子：
9.8
作者：
Bieber,E;Stratman,T;Sanseverino,M;Sangueza,J;Phillippe,M
通讯作者：
Phillippe,M