miRNA Expression Profile in the N2 Phenotype Neutrophils of Colorectal Cancer and Screen of Putative Key miRNAs
miRNA Expression Profile in the N2 Phenotype Neutrophils of Colorectal Cancer and Screen of Putative Key miRNAs
复制标题
结直肠癌 N2 表型中性粒细胞中 miRNA 表达谱及假定关键 miRNA 的筛选
DOI：
10.2147/cmar.s251427
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发表时间：
2020-07
影响因子：
3.3
通讯作者：
Wen-Liang Li
中科院分区：
文献类型：
--
作者：
Liang Wang;Wen-Liang Li
目的：结直肠癌（CRC）是我国最常见的恶性肿瘤之一。该研究的目的是鉴定CRC诊断中的关键基因和mirna，并将其应用于临床。方法：探讨中性粒细胞在原发性结直肠癌组织中的浸润和转移。免疫组化染色观察癌旁组织。诱导后。体外提取含有TGF-β1的N2中性粒细胞、外泌体，并对其进行测序。使用Agilent miRNA芯片筛选miRNA的表达差异。的。将数据导入Web CARMA进行差异表达分析。GO和。使用DIANA-MirPath v3.0, targetscan .数据库进行KEGG富集分析。并将相应的目标导入Gephi进行网络分析。的。利用实时定量聚合酶检测差异表达miRNA的表达水平。结果：共发现2个与N2中性粒细胞相关的mirna，其中2个与N2中性粒细胞相关。hsa-miR-4780表达上调，hsa-miR-3938表达下调。在N2中性粒细胞中下调，与中性粒细胞相比。此外，结果。mirna靶标网络显示hsa-mir-3938和hsa-mir-4780可以调节。TUSC1和ZNF197。hsa-miR-4780和hsa-miR-3938的表达水平。根据RT-PCR结果进行验证。结论：hsa-mir-3938和hsa-mir-4780在N2组间表达差异。中性粒细胞和中性粒细胞。此外，它们对TUSC1和ZNF197的调控作用。DEmiRNA为N2型中性粒细胞的作用机制奠定了理论基础。调节结直肠癌细胞的侵袭和转移，并提供潜在的生物标志物。为临床治疗CRC的预后提供参考。
Objective: Colorectal cancer (CRC) is one of the most common malignant tumors in.the digestive tract, which accounts for 10% of all the malignant tumors in the world..The aim of this study was to identify key genes and miRNAs in CRC diagnosis,.prognosis, and therapy and to further explore the potential molecular mechanisms of.CRC..Methods: The infiltration and metastasis of neutrophils in primary colorectal cancer tissue.and paracancerous tissue were observed by immunohistochemical staining. After inducing.N2 neutrophils with TGF-β1 in vitro, exosomes were extracted and sequenced, and then the.expression differences of miRNAs were screened by using Agilent miRNA microarrays. The.data were imported to the Web CARMA for differential expression analysis. The GO and.KEGG enrichment analysis were performed using DIANA-MirPath v3.0 using TargetScan.database. And the corresponding targets were imported into Gephi for network analysis. The.expression level of differentially expressed miRNA using quantitative real-time polymerase.chain reaction (RT-PCR) was validated..Results: A total of 2 miRNAs were found to be associated with N2 neutrophils, in which the.expression of hsa-miR-4780 was upregulated and the expression of hsa-miR-3938 was.downregulated in N2 neutrophils, compared with the neutrophils. In addition, the results of.miRNA-targets networks showed that the hsa-mir-3938 and hsa-mir-4780 could regulate.TUSC1 and ZNF197. The expression level of hsa-miR-4780 and hsa-miR-3938.wase validated in accordance with the results of RT-PCR..Conclusion: The hsa-mir-3938 and hsa-mir-4780 were differentially expressed between N2.neutrophils and neutrophils. Moreover, the regulation of TUSC1 and ZNF197 by these.DEmiRNA established the theoretical basis for the mechanism of N2 type neutrophils.regulating the invasion and metastasis of CRC cells and provided the potential biomarker.for prognosis for clinical treatment of CRC.
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TUSC1是一种推定的肿瘤抑制基因，可在体外减少肿瘤细胞的生长和体内肿瘤的生长。
影响因子：
3.7
作者：
Shan Z;Shakoori A;Bodaghi S;Goldsmith P;Jin J;Wiest JS
通讯作者：
Wiest JS
DOI：
10.5114/wo.2013.39995
发表时间：
2014
期刊：
Contemporary oncology (Poznan, Poland)
影响因子：
--
作者：
Swiderska M;Choromańska B;Dąbrowska E;Konarzewska-Duchnowska E;Choromańska K;Szczurko G;Myśliwiec P;Dadan J;Ladny JR;Zwierz K
通讯作者：
Zwierz K
肿瘤源性外泌体诱导中性粒细胞N2极化促进胃癌细胞迁移
影响因子：
37.3
作者：
Zhang X;Shi H;Yuan X;Jiang P;Qian H;Xu W
通讯作者：
Xu W
影响因子：
2.5
作者：
Akimitsu Tanio;H. Saito;Chihiro Uejima;S. Takaya;Manabu Yamamoto;Naruo Tokuyasu;Teruhisa Sakamoto;S. Honjo;K. Ashida;Y. Fujiwara
通讯作者：
Akimitsu Tanio;H. Saito;Chihiro Uejima;S. Takaya;Manabu Yamamoto;Naruo Tokuyasu;Teruhisa Sakamoto;S. Honjo;K. Ashida;Y. Fujiwara
影响因子：
8
作者：
Shan, ZH;Parker, T;Wiest, JS
通讯作者：
Wiest, JS