Suppression of p53 potentiates chemosensitivity in nutrient-deprived cholangiocarcinoma cells via inhibition of autophagy.
Suppression of p53 potentiates chemosensitivity in nutrient-deprived cholangiocarcinoma cells via inhibition of autophagy.
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DOI：
10.3892/ol.2017.6449
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发表时间：
2017-08
期刊：
影响因子：
2.9
通讯作者：
Wei LX
中科院分区：
文献类型：
--
作者：
Hu F;Guo XL;Zhang SS;Zhao QD;Li R;Xu Q;Wei LX
Tumor protein p53 has been intensively studied as a major tumor suppressor. The activation of p53 is associated with various anti-neoplastic functions, including cell senescence, cell cycle arrest, apoptosis and inhibition of angiogenesis. However, the role of p53 in cancer cell chemosensitivity remains unknown. Cholangiocarcinoma cell lines QBC939 and RBE were grown in full-nutrient and nutrient-deprived conditions. The cell lines were treated with 5-fluorouracil or cisplatin and the rate of cell death was determined in these and controls using Cell Counting Kit-8 and microscopy-based methods, including in the presence of autophagy inhibitor 3MA, p53 inhibitor PFT-α or siRNA against p53 or Beclin-1. The present study demonstrated that the inhibition of p53 enhanced the sensitivity to chemotherapeutic agents in nutrient-deprived cholangiocarcinoma cells. Nutrient deprivation-induced autophagy was revealed to be inhibited following inhibition of p53. These data indicate that p53 is important for the activation of autophagy in nutrient-deprived cholangiocarcinoma cells, and thus contributes to cell survival and chemoresistance.
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影响因子：
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作者：
Ávalos Y;Canales J;Bravo-Sagua R;Criollo A;Lavandero S;Quest AF
通讯作者：
Quest AF
影响因子：
9.7
作者：
Liu, Juan;Zhang, Cen;Hu, Wenwei;Feng, Zhaohui
通讯作者：
Feng, Zhaohui
影响因子：
13.3
作者：
Lin WJ;Kuang HY
通讯作者：
Kuang HY
DOI：
10.1080/15384101.2015.1121326
发表时间：
2016
期刊：
Cell cycle (Georgetown, Tex.)
影响因子：
--
作者：
Borodkina AV;Shatrova AN;Deryabin PI;Grukova AA;Nikolsky NN;Burova EB
通讯作者：
Burova EB
影响因子：
--
作者：
Vaughan, Catherine;Pearsall, Isabella;Deb, Sumitra
通讯作者：
Deb, Sumitra