Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla.
Identification and characterization of miRNAome and target genes in Pseudostellaria heterophylla.
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太子参 miRNAome 和靶基因的鉴定和表征
DOI：
10.1371/journal.pone.0275566
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
miRNAs通过在转录后水平抑制靶基因的功能，在植物的发育和生长中发挥重要作用。然而，太子参中的miRNAs尚未报道，其在器官形态发生中的功能尚不清楚。本研究共鉴定出159个保守的miRNAs（属于64个家族）和303个水平的miRNAs。其中一些基因在不同组织中特异性上调或下调表达，并靶向了许多参与植物-病原体互作和植物MAPK信号通路的单基因。30个miRNAs与其靶基因（37个unigenes）的表达谱呈显著负相关。此外，大量涉及生长素、玉米素、脱落酸和茉莉酸信号转导的基因被靶向。通过5′RLM-RACE验证两种miRNAs预测的靶点。来自四种病原体的大量mRNA被来自异叶侧耳的miRNA靶向，并且其中一些被miR 414靶向。综上所述，本研究通过高通量测序技术，获得了一组来自4种不同营养组织的miRNAs，并结合构建的转录组进行了分析。这些结果有助于解释miRNAs在器官形态建成和病原菌防御中的作用，为异叶侧耳的育种和遗传改良提供理论依据。
miRNAs play a crucial role in the development and growth of plants by inhibiting the function of targeted genes at the post-transcription level. However, no miRNAs in Pseudostellaria heterophylla have been reported and their function in the morphogenesis of organs is still unclear. In this study, a total of 159 conserved miRNAs (belonging to 64 families) and 303 level miRNAs were identified from P. heterophylla. Some of them showed specifically up or down-regulated expression in different tissues and numbers of unigenes involved in Plant-pathogen interaction and MAPK signaling pathway-plant were targeted. The significant negative correlation of expression profiles between 30 miRNAs and their target genes (37 unigenes) was observed, respectively. Further, a large number of genes involved with signal transduction of auxin, zeatin, abscisic acid and, jasmonic acid were targeted. Predicated targets of two miRNAs were validated by 5′RLM-RACE, respectively. A large number of mRNAs from four pathogens were targeted by miRNAs from P. heterophylla and some of them were targeted by miR414. In summary, we reported a population of miRNAs from four different vegetative tissues of P. heterophylla by high throughput sequencing, which was analyzed by combining with the constructed transcriptome. These results may help to explain the function of miRNAs in the morphogenesis of organs and defense of pathogens, and may provide theoretical basis for breeding and genetic improvement of P. heterophylla.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
DOI：
10.3390/molecules16043360
发表时间：
2011-04-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Pang W;Lin S;Dai Q;Zhang H;Hu J
通讯作者：
Hu J
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
11.6
作者：
Guo, HS;Xie, Q;Chua, NH
通讯作者：
Chua, NH
影响因子：
64.5
作者：
LEE, RC;FEINBAUM, RL;AMBROS, V
通讯作者：
AMBROS, V