FishMicrosat: a microsatellite database of commercially important fishes and shellfishes of the Indian subcontinent.

FishMicrosat: a microsatellite database of commercially important fishes and shellfishes of the Indian subcontinent.
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DOI:
10.1186/1471-2164-14-630
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发表时间:
2013-09-18
期刊:
影响因子:
4.4
通讯作者:
Sarkar UK
Sarkar UK
中科院分区:
生物学2区
文献类型:
--
作者:
Nagpure NS;Rashid I;Pati R;Pathak AK;Singh M;Singh SP;Sarkar UK

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微卫星DNA是构建遗传连锁图谱和进行群体遗传学研究的重要遗传标记之一。公共领域的生物数据库中包含了包括鱼类在内的许多生物体的大量微卫星序列。从这些数据来源中提取微卫星数据,并将其纳入一个数据库,以便于进行序列分析和浏览相关信息。该系统还有助于设计重复基因座侧翼区的引物序列,用于群体内多态性的PCR鉴定。FishMicrosat是一个鱼类和贝类微卫星序列数据库,包括重要的水产养殖物种,如Lates calcarifer,Ctenopharyngodon idella,Hypophthalmichthys molitrix,Penaeus monodon,Labeo rohita,Oreochromis niloticus,Fenneropenaeus indicus和Macrobrachium rosenbertus。该数据库包含来自印度次大陆的41个物种的4398个微卫星序列,隶属于15个科。以NCBI的GenBank作为数据库的主要数据源。该数据库提供有关简单和复合微卫星,它们的集群和序列内的基因座方向的信息。该数据库已与网络界面中的不同工具集成,如引物设计,基因座查找,映射重复,检测跨物种序列之间的相似性,以及使用基序和关键字进行搜索。此外,该数据库还能够通过记录概览浏览关于前10个科和前10个物种的信息。FishMicrosat数据库是鱼类和贝类微卫星分析和跨物种位点识别的有用资源,在种群遗传学、进化研究和物种间遗传相关性研究中具有重要应用。该数据库可进一步扩展,以包括来自其他区域的鱼类和贝类的微卫星数据以及关于重要水产养殖物种的基因组测序计划的现有信息。
Microsatellite DNA is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. The biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. The microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. The system also helps to design primer sequences for flanking regions of repeat loci for PCR identification of polymorphism within populations. FishMicrosat is a database of microsatellite sequences of fishes and shellfishes that includes important aquaculture species such as Lates calcarifer, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Penaeus monodon, Labeo rohita, Oreochromis niloticus, Fenneropenaeus indicus and Macrobrachium rosenbergii. The database contains 4398 microsatellite sequences of 41 species belonging to 15 families from the Indian subcontinent. GenBank of NCBI was used as a prime data source for developing the database. The database presents information about simple and compound microsatellites, their clusters and locus orientation within sequences. The database has been integrated with different tools in a web interface such as primer designing, locus finding, mapping repeats, detecting similarities among sequences across species, and searching using motifs and keywords. In addition, the database has the ability to browse information on the top 10 families and the top 10 species, through record overview. FishMicrosat database is a useful resource for fish and shellfish microsatellite analyses and locus identification across species, which has important applications in population genetics, evolutionary studies and genetic relatedness among species. The database can be expanded further to include the microsatellite data of fishes and shellfishes from other regions and available information on genome sequencing project of species of aquaculture importance.
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