Chromosome-level genome assembly for the horned-gall aphid provides insights into interactions between gall-making insect and its host plant.
Chromosome-level genome assembly for the horned-gall aphid provides insights into interactions between gall-making insect and its host plant.
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DOI：
10.1002/ece3.8815
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发表时间：
2022-04
影响因子：
2.6
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
--
作者：
Wei, Hong-Yuan;Ye, Yu-Xuan;Huang, Hai-Jian;Chen, Ming-Shun;Yang, Zi-Xiang;Chen, Xiao-Ming;Zhang, Chuan-Xi
中华扁蚜是一种重要的经济昆虫，其角瘿在医药、化工等领域具有重要的应用价值。到目前为止，已经报道了20多个蚜虫基因组。大多数测序的基因组来自自由生活的蚜虫。在这里，我们从一个恼人的蚜虫中产生了一个高质量的基因组组装。最后的基因组组装是271.52 Mb，代表最小的蚜虫基因组测序之一。基因组组装基于重叠群，基因组序列的支架N50值分别为3.77 Mb和20.41 Mb。97%的组装序列锚定在13条染色体上。基于BUSCO分析，该组装涉及96.9%的保守节肢动物和98.5%的保守半翅目单拷贝直向同源基因。共预测了14，089个蛋白质编码基因。系统发育分析表明，S.中华绒螯蟹在大约5700万年前（MYA）从棉蚜的共同祖先分化而来。此外，编码唾液腺蛋白的35个基因在S. chinensis形成了一个瘿，这表明它们在瘿的形成和植物防御抑制中具有潜在的作用。综合来看，这款高品质的S.中国蚜虫基因组的组装和注释为进一步揭示蚜虫瘿的形成机制和探讨蚜虫与寄主植物的相互作用提供了坚实的遗传基础。中华扁蚜（Schlechtendalia chinensis）的基因组为271.52 Mb。97%的组装序列锚定在13条染色体上。共预测了14，089个蛋白质编码基因，其中35个编码唾液腺蛋白的基因可能在植物瘿形成和防御抑制中发挥作用。
The aphid Schlechtendalia chinensis is an economically important insect that can induce horned galls, which are valuable for the medicinal and chemical industries. Up to now, more than twenty aphid genomes have been reported. Most of the sequenced genomes are derived from free‐living aphids. Here, we generated a high‐quality genome assembly from a galling aphid. The final genome assembly is 271.52 Mb, representing one of the smallest sequenced genomes of aphids. The genome assembly is based on contig and scaffold N50 values of the genome sequence are 3.77 Mb and 20.41 Mb, respectively. Nine‐seven percent of the assembled sequences was anchored onto 13 chromosomes. Based on BUSCO analysis, the assembly involved 96.9% of conserved arthropod and 98.5% of the conserved Hemiptera single‐copy orthologous genes. A total of 14,089 protein‐coding genes were predicted. Phylogenetic analysis revealed that S. chinensis diverged from the common ancestor of Eriosoma lanigerum approximately 57 million years ago (MYA). In addition, 35 genes encoding salivary gland proteins showed differentially when S. chinensis forms a gall, suggesting they have potential roles in gall formation and plant defense suppression. Taken together, this high‐quality S. chinensis genome assembly and annotation provide a solid genetic foundation for future research to reveal the mechanism of gall formation and to explore the interaction between aphids and their host plants. The genome of a galling aphid Schlechtendalia chinensis is 271.52 Mb. Nine‐seven percent of the assembled sequences were anchored onto 13 chromosomes. A total of 14,089 protein‐coding genes were predicted and 35 genes encoding salivary gland proteins have potential roles in gall formation and plant defense suppression.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1016/j.cub.2021.01.104
发表时间：
2021-05-10
期刊：
Current biology : CB
影响因子：
--
作者：
Korgaonkar A;Han C;Lemire AL;Siwanowicz I;Bennouna D;Kopec RE;Andolfatto P;Shigenobu S;Stern DL
通讯作者：
Stern DL
DOI：
10.1093/jisesa/iey094
发表时间：
2018-09-01
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Al-Jbory Z;El-Bouhssini M;Chen MS
通讯作者：
Chen MS
影响因子：
4.9
作者：
Bao W;Kojima KK;Kohany O
通讯作者：
Kohany O
影响因子：
14.9
作者：
Kalvari I;Argasinska J;Quinones-Olvera N;Nawrocki EP;Rivas E;Eddy SR;Bateman A;Finn RD;Petrov AI
通讯作者：
Petrov AI