A novel family of secreted insect proteins linked to plant gall development.
A novel family of secreted insect proteins linked to plant gall development.
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一个与植物虫瘿发育相关的昆虫分泌蛋白新家族。
DOI：
10.1016/j.cub.2021.01.104
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发表时间：
2021-05-10
期刊：
影响因子：
--
通讯作者：
Stern DL
中科院分区：
文献类型：
--
作者：
Korgaonkar A;Han C;Lemire AL;Siwanowicz I;Bennouna D;Kopec RE;Andolfatto P;Shigenobu S;Stern DL
在一种复杂的物种间利用形式中，许多昆虫劫持了植物的发育，诱导出被称为“瘿”的新型植物器官，为昆虫提供营养来源和临时住所。虫瘿是由昆虫分子驱动的植物细胞生物学重编程的结果，但特定昆虫分子在虫瘿发育中的作用尚未确定。在这里，我们研究了蚜虫，它在维吉尼亚金缕梅的叶子上产生独特的“锥体”瘿。我们发现，蚜虫胆色决定基因（dgc）的衍生遗传变异与蚜虫唾液腺dgc转录的强烈下调、7个参与花青素合成的基因在虫瘿中的上调以及两种红色花青素在虫瘿中的沉积有关。我们假设蚜虫将DGC蛋白注入虫瘿，这导致了少数植物基因的差异表达。Dgc是一个庞大的、多样化的新型预测分泌蛋白家族的成员，其特征是一对间距很大的半胱氨酸-酪氨酸-半胱氨酸（CYC）残基，我们将其命名为BICYCLE蛋白。自行车基因在唾液腺中表达最强烈，特别是在瘿蚜的世代中，这表明它们可能调节瘿发育的许多方面。自行车基因经历了异常频繁的多样化选择，这与它们在蚜虫和寄主植物之间的分子军备竞赛中控制胆发育的潜在作用是一致的。Korgaonkar等人报道了由自行车基因编码的新型分泌蚜虫蛋白。决定胆汁颜色的bicycle基因的变异改变了目标植物基因的表达，表明bicycle蛋白调节了胆汁的发育。
In an elaborate form of inter-species exploitation, many insects hijack plant development to induce novel plant organs called galls that provide the insect with a source of nutrition and a temporary home. Galls result from dramatic reprogramming of plant cell biology driven by insect molecules, but the roles of specific insect molecules in gall development have not yet been determined. Here we study the aphid Hormaphis cornu, which makes distinctive “cone” galls on leaves of witch hazel Hamamelis virginiana. We found that derived genetic variants in the aphid gene determinant of gall color (dgc) are associated with strong downregulation of dgc transcription in aphid salivary glands, upregulation in galls of seven genes involved in anthocyanin synthesis, and deposition of two red anthocyanins in galls. We hypothesize that aphids inject DGC protein into galls, and that this results in differential expression of a small number of plant genes. Dgc is a member of a large, diverse family of novel predicted secreted proteins characterized by a pair of widely spaced cysteine-tyrosine-cysteine (CYC) residues, which we named BICYCLE proteins. Bicycle genes are most strongly expressed in the salivary glands specifically of galling aphid generations, suggesting that they may regulate many aspects of gall development. Bicycle genes have experienced unusually frequent diversifying selection, consistent with their potential role controlling gall development in a molecular arms race between aphids and their host plants. Korgaonkar et al. report on novel secreted aphid proteins encoded by bicycle genes. Variation in the bicycle gene determinant of gall color alters expression of targeted plant genes, suggesting that BICYCLE proteins modulate gall development.
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影响因子：
5.8
作者：
Barnett, Derek W.;Garrison, Erik K.;Marth, Gabor T.
通讯作者：
Marth, Gabor T.
影响因子：
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作者：
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
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通讯作者：
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64.8
作者：
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通讯作者：
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DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
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