Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis.
Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis.
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DOI：
10.1093/jxb/ers315
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发表时间：
2013-01
影响因子：
6.9
通讯作者：
Kochian LV
中科院分区：
文献类型：
--
作者：
Milner MJ;Seamon J;Craft E;Kochian LV
更好地了解拟南芥ZIP家族的微量营养素转运蛋白的作用是必要的，以促进我们对植物锌，铁，锰，铜稳态的理解。在目前的研究中，11个拟南芥ZIP家族成员尚未得到很好的表征，首先筛选他们的能力，以补充四个酵母突变体缺陷的锌，铁，锰，铜吸收。六个拟南芥ZIP基因补充酵母锌摄取缺陷突变体，一个能够部分地补充酵母铁摄取缺陷突变体，六个ZIP家族成员补充锰摄取缺陷突变体，没有补充铜摄取缺陷突变体。AtZIP 1和AtZIP 2被选择用于进一步的研究，因为初步的酵母和植物分析表明它们都可能是根Zn和Mn转运蛋白。在酵母中，AtZIP 1和AtZIP 2都补充了锌和锰摄取突变体，这表明它们都可以运输锌和/或锰。这两个基因的表达是本地化的根中柱，AtZIP 1的表达也被发现在叶脉管系统。AtZIP 1是一种液泡转运蛋白，而AtZIP 2定位于质膜。拟南芥AtZIP 1和AtZIP 2 T-DNA敲除系的功能研究表明，这两种转运蛋白在Mn（可能还有Zn）从根到茎的转运中起作用。AtZIP 1可能在Mn从液泡到根星状细胞的细胞质中起作用，并可能有助于径向移动到木质部薄壁组织。AtZIP 2，另一方面，可能介导Mn（可能还有Zn）吸收到根星状细胞，因此也可能有助于Mn/Zn移动中柱的木质部薄壁组织，随后木质部加载和运输到拍摄。
A better understanding of the role of the Arabidopsis ZIP family of micronutrient transporters is necessary in order to advance our understanding of plant Zn, Fe, Mn, and Cu homeostasis. In the current study, the 11 Arabidopsis ZIP family members not yet well characterized were first screened for their ability to complement four yeast mutants defective in Zn, Fe, Mn, or Cu uptake. Six of the Arabidopsis ZIP genes complemented a yeast Zn uptake-deficient mutant, one was able partially to complement a yeast Fe uptake-deficient mutant, six ZIP family members complemented an Mn uptake-deficient mutant, and none complemented the Cu uptake-deficient mutant. AtZIP1 and AtZIP2 were then chosen for further study, as the preliminary yeast and in planta analysis suggested they both may be root Zn and Mn transporters. In yeast, AtZIP1 and AtZIP2 both complemented the Zn and Mn uptake mutants, suggesting that they both may transport Zn and/or Mn. Expression of both genes is localized to the root stele, and AtZIP1 expression was also found in the leaf vasculature. It was also found that AtZIP1 is a vacuolar transporter, while AtZIP2 is localized to the plasma membrane. Functional studies with Arabidopsis AtZIP1 and AtZIP2 T-DNA knockout lines suggest that both transporters play a role in Mn (and possibly Zn) translocation from the root to the shoot. AtZIP1 may play a role in remobilizing Mn from the vacuole to the cytoplasm in root stellar cells, and may contribute to radial movement to the xylem parenchyma. AtZIP2, on the other hand, may mediate Mn (and possibly Zn) uptake into root stellar cells, and thus also may contribute to Mn/Zn movement in the stele to the xylem parenchyma, for subsequent xylem loading and transport to the shoot.
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影响因子：
11.6
作者：
Vert, G;Grotz, N;Curie, C
通讯作者：
Curie, C
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
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Ecker, JR
影响因子：
7.4
作者：
Hart, JJ;Welch, RM;Kochian, LV
通讯作者：
Kochian, LV
DOI：
10.1073/pnas.97.9.4991
发表时间：
2000-04-25
影响因子：
11.1
作者：
Thomine, S;Wang, RC;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
4.8
作者：
Peña, MMO;Puig, S;Thiele, DJ
通讯作者：
Thiele, DJ