Automated 3-D Printed Arrays to Evaluate Genotoxic Chemistry: E-Cigarettes and Water Samples.
Automated 3-D Printed Arrays to Evaluate Genotoxic Chemistry: E-Cigarettes and Water Samples.
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DOI：
10.1021/acssensors.7b00118
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发表时间：
2017-05-26
期刊：
影响因子：
8.9
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Kadimisetty K;Malla S;Rusling JF
开发了一种新型的、自动化的、低成本的、三维（3-D）打印的微流控阵列，用于检测环境样品中化学物质代谢产物对DNA的损伤。电致发光（ECL）检测平台在10 nm深的微孔中整合了微粒体酶、DNA和发射ECL的钌金属聚合物的逐层（LbL）组装膜。液体样品被引入到阵列中，由人类酶代谢，如果可能的话，产物与DNA反应，并且通过带有相机的ECL检测DNA损伤。通过阵列测量相对DNA损伤评估样品的遗传毒性潜力。该阵列在5分钟内同时分析三个样品。香烟和电子烟烟雾提取物和污染水样品的测量用于建立概念验证。电子烟蒸气的潜在遗传毒性反应与传统香烟的烟雾相似。未经处理的废水显示出很高的遗传毒性潜力相比，从污染控制处理厂处理的废水可以忽略不计的值。测量已知产生高速率代谢物相关DNA损伤的化学品的反应性，并将环境样品的阵列结果表示为来自这些标准的等效响应，以评估可能的DNA损伤的严重程度。在处理过程中对废水样品进行遗传毒性评估也突出了今后的现场监测应用。
A novel, automated, low cost, three-dimensional (3-D) printed microfluidic array was developed to detect DNA damage from metabolites of chemicals in environmental samples. The electrochemiluminescent (ECL) detection platform incorporates layer-by-layer (LbL) assembled films of microsomal enzymes, DNA and an ECL-emitting ruthenium metallopolymer in ∼10 nm deep microwells. Liquid samples are introduced into the array, metabolized by the human enzymes, products react with DNA if possible, and DNA damage is detected by ECL with a camera. Measurements of relative DNA damage by the array assess the genotoxic potential of the samples. The array analyzes three samples simultaneously in 5 min. Measurement of cigarette and e-cigarette smoke extracts and polluted water samples was used to establish proof of concept. Potentially genotoxic reactions from e- cigarette vapor similar to smoke from conventional cigarettes were demonstrated. Untreated wastewater showed a high genotoxic potential compared to negligible values for treated wastewater from a pollution control treatment plant. Reactivity of chemicals known to produce high rates of metabolite-related DNA damage were measured, and array results for environmental samples were expressed in terms of equivalent responses from these standards to assess severity of possible DNA damage. Genotoxic assessment of wastewater samples during processing also highlighted future on-site monitoring applications.
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影响因子：
2.1
作者：
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通讯作者：
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影响因子：
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影响因子：
8.9
作者：
Bishop GW;Satterwhite-Warden JE;Bist I;Chen E;Rusling JF
通讯作者：
Rusling JF