The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family.
The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family.
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茄科植物可以感知青枯罗尔斯顿菌 csp22 肽，但不能感知鞭毛蛋白衍生肽
DOI：
10.1111/pbi.12874
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发表时间：
2018-07
影响因子：
13.8
通讯作者：
Macho AP
中科院分区：
文献类型：
--
作者：
Wei Y;Caceres-Moreno C;Jimenez-Gongora T;Wang K;Sang Y;Lozano-Duran R;Macho AP
青枯菌是青枯病的病原菌，因其致死性强、寄主范围广、持久性强、地理分布广而被认为是最具破坏性的细菌病原菌之一。尽管在过去的几年里对植物免疫进行了广泛的研究，但对青枯病菌激活植物免疫的分子模式的理解仍然很少，这阻碍了对青枯病抗性策略的发展。对细菌鞭毛蛋白保守多肽flg22的感知被认为是植物感知入侵细菌的范例；然而，尚未检测到青枯病菌flg22的激发子活性。最近的报道表明，鞭毛蛋白的其他表位能够在茄科植物中的特定物种中引发免疫反应，但我们的结果表明，这些植物没有察觉到青枯菌鞭毛蛋白的任何表位。在对青枯菌激发子多肽的搜索中，我们发现了几个与激发子多肽csp22相似的蛋白质序列，据报道，csp22在特定的茄科植物中诱导免疫。青枯菌csp22多肽(Csp22Rsol)在烟草和番茄中确实能激发免疫反应，但在拟南芥中不能。此外，csp22Rsol处理提高了番茄对青枯病的抗性。表达番茄csp22受体(SlCORE)的转基因青枯草植株获得了对csp22Rsol的反应能力，并对青枯病菌的侵染产生了更强的抗性。我们的结果揭示了植物感知青枯病菌的机制，为改进目前产生对青枯病菌抗性的方法铺平了道路。
Ralstonia solanacearum, the causal agent of bacterial wilt disease, is considered one of the most destructive bacterial pathogens due to its lethality, unusually wide host range, persistence and broad geographical distribution. In spite of the extensive research on plant immunity over the last years, the perception of molecular patterns from R. solanacearum that activate immunity in plants is still poorly understood, which hinders the development of strategies to generate resistance against bacterial wilt disease. The perception of a conserved peptide of bacterial flagellin, flg22, is regarded as paradigm of plant perception of invading bacteria; however, no elicitor activity has been detected for R. solanacearum flg22. Recent reports have shown that other epitopes from flagellin are able to elicit immune responses in specific species from the Solanaceae family, yet our results show that these plants do not perceive any epitope from R. solanacearum flagellin. Searching for elicitor peptides from R. solanacearum, we found several protein sequences similar to the consensus of the elicitor peptide csp22, reported to elicit immunity in specific Solanaceae plants. A R. solanacearum csp22 peptide (csp22Rsol) was indeed able to trigger immune responses in Nicotiana benthamiana and tomato, but not in Arabidopsis thaliana. Additionally, csp22Rsol treatment conferred increased resistance to R. solanacearum in tomato. Transgenic A. thaliana plants expressing the tomato csp22 receptor (SlCORE) gained the ability to respond to csp22Rsol and became more resistant to R. solanacearum infection. Our results shed light on the mechanisms for perception of R. solanacearum by plants, paving the way for improving current approaches to generate resistance against R. solanacearum.
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影响因子：
3.5
作者：
Lu, Haibin;Lema, Saul A.;Coll, Nuria S.
通讯作者：
Coll, Nuria S.
影响因子：
3.2
作者：
McGarvey, JA;Denny, TP;Schell, MA
通讯作者：
Schell, MA
影响因子：
7.2
作者：
Gómez-Gómez, L;Felix, G;Boller, T
通讯作者：
Boller, T
DOI：
10.1111/nph.12408
发表时间：
2013-11
期刊：
The New phytologist
影响因子：
--
作者：
Clarke CR;Chinchilla D;Hind SR;Taguchi F;Miki R;Ichinose Y;Martin GB;Leman S;Felix G;Vinatzer BA
通讯作者：
Vinatzer BA
影响因子：
5.6
作者：
Huet G
通讯作者：
Huet G