New 1,2-Dihydropyridine-Based Fluorophores and Their Applications as Fluorescent Probes.
New 1,2-Dihydropyridine-Based Fluorophores and Their Applications as Fluorescent Probes.
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DOI：
10.1021/acsomega.7b01835
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发表时间：
2018-01-31
期刊：
影响因子：
4.1
通讯作者：
Lankalapalli RS
中科院分区：
文献类型：
--
作者：
Jamsheena V;Mishra RK;Veena KS;Sini S;Jayamurthy P;Lankalapalli RS
设计并合成了新的1,2-二氢吡啶（1,2- dhp）基荧光团1a-1h，以二氨基二酸酯、醛和三氟乙酸介导的原位生成的腙为原料，采用一锅四组分缩合反应合成。对1,2- dhps的光物理性质进行了详细的研究，发现部分dhps在HeLa细胞系（宫颈癌细胞）中表现出选择性线粒体染色能力。详细的光物理研究导致1,2- dhp 1h设计为最佳的荧光团，适合其作为水介质中的小分子探针的潜在应用。此外，1,2- dhp 1h在长波长区域（λem≈600 nm）的发射强度比其磷酸化类似物1h ‘增强了6倍，这使得1,2- dhp 1h ’满足作为蛋白酪氨酸磷酸酶的生物探针的要求，如在L6肌肉细胞裂解液中所示。
New 1,2-dihydropyridine (1,2-DHP)-based fluorophores 1a–1h were designed and synthesized by a one-pot four-component condensation reaction using dienaminodioate, aldehydes, and an in situ-generated hydrazone mediated by trifluoroacetic acid. The photophysical properties of 1,2-DHPs were studied in detail, and a few of them exhibited selective mitochondrial staining ability in HeLa cell lines (cervical cancer cells). A detailed photophysical investigation led to the design of 1,2-DHP 1h as an optimal fluorophore suitable for its potential application as a small molecule probe in the aqueous medium. Also, 1,2-DHP 1h exhibited sixfold enhanced emission intensity than its phosphorylated analogue 1h′ in the long wavelength region (λem ≈ 600 nm), which makes 1,2-DHP 1h′ meet the requirement as a bioprobe for protein tyrosine phosphatases, shown in L6 muscle cell lysate.
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影响因子：
14.8
作者：
Arrowsmith CH;Audia JE;Austin C;Baell J;Bennett J;Blagg J;Bountra C;Brennan PE;Brown PJ;Bunnage ME;Buser-Doepner C;Campbell RM;Carter AJ;Cohen P;Copeland RA;Cravatt B;Dahlin JL;Dhanak D;Edwards AM;Frederiksen M;Frye SV;Gray N;Grimshaw CE;Hepworth D;Howe T;Huber KV;Jin J;Knapp S;Kotz JD;Kruger RG;Lowe D;Mader MM;Marsden B;Mueller-Fahrnow A;Müller S;O'Hagan RC;Overington JP;Owen DR;Rosenberg SH;Roth B;Ross R;Schapira M;Schreiber SL;Shoichet B;Sundström M;Superti-Furga G;Taunton J;Toledo-Sherman L;Walpole C;Walters MA;Willson TM;Workman P;Young RN;Zuercher WJ
通讯作者：
Zuercher WJ
影响因子：
2.9
作者：
Królicki, R;Jarzeba, W;Lampre, I
通讯作者：
Lampre, I
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru
影响因子：
1.8
作者：
Challa, Chandrasekhar;John, Manju;Lankalapalli, Ravi S.
通讯作者：
Lankalapalli, Ravi S.
影响因子：
4.4
作者：
BECKE, AD
通讯作者：
BECKE, AD