Transcriptomic evidence for a dramatic functional transition of the malpighian tubules after a blood meal in the Asian tiger mosquito Aedes albopictus.
Transcriptomic evidence for a dramatic functional transition of the malpighian tubules after a blood meal in the Asian tiger mosquito Aedes albopictus.
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DOI：
10.1371/journal.pntd.0002929
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发表时间：
2014-06
影响因子：
3.8
通讯作者：
Piermarini PM
中科院分区：
文献类型：
--
作者：
Esquivel CJ;Cassone BJ;Piermarini PM
成年雌蚊消耗脊椎动物的血液是其繁殖所必需的，但它也对蚊子的渗透调节和代谢提出了重大的生理挑战。蚊子的肾（马尔比氏）小管通过排出摄入的多余水分和盐，在血液初始处理过程中发挥关键作用。然而，目前尚不清楚这些小管如何参与血液消化过程中产生的废物（如血红素、氨）的代谢和排泄。在这里，我们使用RNA-Seq检测了高度侵袭性亚洲虎蚊白纹伊蚊（Aedes albopictus）在食用血餐后的头24小时内马尔比氏小管转录本表达的全球变化。我们发现，在吸血后3小时、12小时和24小时，从蚊子身上分离的马尔比氏小管的转录组发生了渐进式的全局变化。值得注意的是，对差异表达转录本的DAVID功能聚类分析显示，1)与氧化代谢、主动运输和mRNA翻译相关的转录本下调，2)与抗氧化剂和解毒、蛋白质水解活性、氨基酸代谢和细胞骨架动力学相关的转录本上调。结果表明，血液喂养引起了上皮的功能转变，从一个专门从事活跃的上皮液体分泌（如利尿）到一个专门从事解毒和代谢废物排泄的上皮。我们的研究结果首次揭示了蚊子马尔比氏小管在慢性血液加工中的作用。亚洲虎蚊白纹伊蚊是几种医学上重要的虫媒病毒的媒介，也是世界上最具侵入性的蚊子之一。目前，针对蚊子的现有控制措施正受到针对神经系统的杀虫剂出现抗药性的挑战。因此，有必要寻找新的生理靶点来指导新型杀虫剂的开发。我们最近证明，蚊子的“肾脏”（马尔比氏小管）为杀虫剂提供了一个有价值的、新的生理靶点。然而，我们对这种组织如何促进蚊子对血液的慢性代谢过程的理解是有限的。在这里，我们描述了成年雌性白纹伊蚊马尔比氏小管中转录物表达的变化，目的是确定可能揭示杀虫剂开发有价值靶点的关键分子途径。我们发现了马尔比氏小管转录本积累的巨大变化，这为1)了解马尔比氏小管在血食后的潜在功能作用提供了新的见解；2)揭示了新的潜在分子途径和靶点，指导开发破坏蚊子肾功能的新型杀虫剂。
The consumption of a vertebrate blood meal by adult female mosquitoes is necessary for their reproduction, but it also presents significant physiological challenges to mosquito osmoregulation and metabolism. The renal (Malpighian) tubules of mosquitoes play critical roles in the initial processing of the blood meal by excreting excess water and salts that are ingested. However, it is unclear how the tubules contribute to the metabolism and excretion of wastes (e.g., heme, ammonia) produced during the digestion of blood. Here we used RNA-Seq to examine global changes in transcript expression in the Malpighian tubules of the highly-invasive Asian tiger mosquito Aedes albopictus during the first 24 h after consuming a blood meal. We found progressive, global changes in the transcriptome of the Malpighian tubules isolated from mosquitoes at 3 h, 12 h, and 24 h after a blood meal. Notably, a DAVID functional cluster analysis of the differentially-expressed transcripts revealed 1) a down-regulation of transcripts associated with oxidative metabolism, active transport, and mRNA translation, and 2) an up-regulation of transcripts associated with antioxidants and detoxification, proteolytic activity, amino-acid metabolism, and cytoskeletal dynamics. The results suggest that blood feeding elicits a functional transition of the epithelium from one specializing in active transepithelial fluid secretion (e.g., diuresis) to one specializing in detoxification and metabolic waste excretion. Our findings provide the first insights into the putative roles of mosquito Malpighian tubules in the chronic processing of blood meals. The Asian tiger mosquito Aedes albopictus is a vector of several medically-important arboviruses and one of the most invasive mosquito species in the world. Existing control measures for mosquitoes are presently being challenged by the emergence of resistance to insecticides that target the nervous system. Thus, it is necessary to identify novel physiological targets to guide the development of new insecticides. We recently demonstrated that the ‘kidneys’ (Malpighian tubules) of mosquitoes offer a valuable, new physiological target for insecticides. However, our understanding of how this tissue contributes to the chronic metabolic processing of blood meals by mosquitoes is limited. Here we characterize the changes in transcript expression that occur in the Malpighian tubules of adult female A. albopictus with the goal of identifying key molecular pathways that may reveal valuable targets for insecticide development. We find dramatic changes in transcript accumulation in Malpighian tubules, which 1) provide new insights into the potential functional roles of Malpighian tubules after a blood meal, and 2) reveal new potential molecular pathways and targets to guide the development of new insecticides that would disrupt the renal functions of mosquitoes.
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影响因子：
64.8
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4.4
作者：
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Collins FH
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