Circulating MicroRNA-486 and MicroRNA-146a serve as potential biomarkers of sarcopenia in the older adults.
Circulating MicroRNA-486 and MicroRNA-146a serve as potential biomarkers of sarcopenia in the older adults.
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DOI：
10.1186/s12877-021-02040-0
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发表时间：
2021-01-30
期刊：
影响因子：
4.1
通讯作者：
Wang JS
中科院分区：
文献类型：
--
作者：
Liu HC;Han DS;Hsu CC;Wang JS
与年龄相关的骨质疏松症显著增加了老年人功能受限和死亡的风险。尽管循环microRNAs(c-miRNAs)与衰老相关的细胞衰老和炎症有关，但c-miRNAs与老年人骨骼肌减少之间的关系尚不清楚。这项研究调查了循环中的myo-miRNAs和炎症相关的miRNAs是否与老年人的骨骼肌减少有关。本研究从597名社区老年人中招募了77名符合条件的受试者(男41名，女36名)，并将其分为正常组(n = 24例)、运动失调组(无肿块时肌肉功能丧失，n = 35例)和骨质疏松症(伴肿块时肌肉功能丧失组，n = 18例)。采用定量聚合酶链式反应和酶联免疫吸附试验分别检测肌型(c-miRNA-133a和c-miRNA-486)和炎症相关(c-miRNA-21和c-miRNA-146a)相关miRNAs，以及炎症相关细胞因子和过氧化产物水平。骨质疏松组的骨骼肌质量指数(SMI)、握力、步速以及c-miR-486和c-miR-146a水平均低于正常组和营养不良组。C-miR-486水平与SMI呈正相关(r = 0.334，P = 0.003)，c-miR-146a水平与SMI(r = 0.240，P = 0.035)、握力(r = 0.253，P = 0.027)呈正相关。在受试者操作特征分析中，c-miR-486和c-miR-146a的曲线下面积分别为0.708(95%可信区间：0.561~0.855，P = 0.008)和0.676(95%CI：0.551~0.801，P = 0.024)。然而，SMI/握力/步速与血浆髓过氧化物酶/白介素1훽/白介素6水平之间没有明显的相关性。Myo-miRNA(c-miR-486)和炎症相关miRNA(c-miR-146a)作为老年性骨质疏松症的重要生物标志物优于血浆中的炎性过氧化物酶/细胞因子。
Age-related sarcopenia meaningfully increases the risks of functional limitations and mortality in the older adults. Although circulating microRNAs (c-miRNAs) are associated with aging-related cellular senescence and inflammation, the relationships between c-miRNAs and sarcopenia in the older adults remain unclear. This study investigates whether circulating myo-miRNAs and inflammation-related miRNAs are associated with sarcopenia in the older adults. This investigation recruited 77 eligible subjects (41 males and 36 females) from 597 community-dwelling older adults, and then divided them into normal (n = 24), dynapenic (loss of muscular function without mass, n = 35), and sarcopenic groups (loss of muscular function with mass, n = 18). Moreover, myo- (c-miRNA-133a and c-miRNA-486) and inflammation- (c-miRNA-21 and c-miRNA-146a) related miRNAs, as well as, inflammatory-related cytokine and peroxide levels in plasma were determined using quantitative polymerase chain reaction and ELISA, respectively. Sarcopenic group exhibited lesser skeletal muscle mass index (SMI), handgrip strength, and gait speed, as well as, lower c-miR-486 and c-miR-146a levels, compared to those of normal and dynapenic groups. Moreover, c-miR-486 level was positively related to SMI (r = 0.334, P = 0.003), whereas c-miR-146a level was positively associated with SMI (r = 0.240, P = 0.035) and handgrip strength (r = 0.253, P = 0.027). In the receiver operating characteristic analysis for predicting sarcopenia, the area under the curve in c-miR-486 was 0.708 (95% confidence interval: 0.561–0.855, P = 0.008) and c-miR-146a was 0.676 (95% CI: 0.551–0.801, P = 0.024). However, no significant relationships were observed between SMI/handgrip strength/gait speed and plasma myeloperoxidase/interleukin-1훽/interleukin-6 levels. Myo-miRNA (c-miR-486) and inflammation-related miRNA (c-miR-146a) are superior to inflammatory peroxide/cytokines in plasma for serving as critical biomarkers of age-related sarcopenia.
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DOI：
10.1261/rna.044149.113
发表时间：
2014-09
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Cornett AL;Lutz CS
通讯作者：
Lutz CS
一种用于设计基于茎环的定量PCR测定的多功能方法，用于检测小调节RNA分子。
影响因子：
3.7
作者：
Czimmerer Z;Hulvely J;Simandi Z;Varallyay E;Havelda Z;Szabo E;Varga A;Dezso B;Balogh M;Horvath A;Domokos B;Torok Z;Nagy L;Balint BL
通讯作者：
Balint BL
DOI：
10.1111/j.1532-5415.2011.03779.x
发表时间：
2012-01-01
影响因子：
6.3
作者：
Stenholm, Sari;Tiainen, Kristina;Koskinen, Seppo
通讯作者：
Koskinen, Seppo
影响因子：
--
作者：
Singer JW;Fleischman A;Al-Fayoumi S;Mascarenhas JO;Yu Q;Agarwal A
通讯作者：
Agarwal A
影响因子：
5.3
作者：
Vasa-Nicotera, Mariuca;Chen, Hailan;Federici, Massimo
通讯作者：
Federici, Massimo