Highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples.
Highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples.
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DOI：
10.1371/journal.pone.0054005
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Morais PV
中科院分区：
文献类型：
--
作者：
Branco R;Cristóvão A;Morais PV
微生物生物报告提供了良好的潜力，用于检测污染环境中的污染物的生物可利用部分，这是目前不能很容易地测量。本文介绍了两种微生物生物报告器的构建和评价，旨在检测污染水样中的生物可利用的铬酸盐。开发的生物报告基因是基于绿色荧光蛋白的表达控制下的chr启动子和chrB调节基因的TnOtChr决定簇从苍白杆菌5bvl1。pCHRGFP1大肠杆菌报告基因被证明是特异性和敏感性的，最低可检测浓度为100 nM铬酸盐，不与其他重金属或分析的化合物反应。为了使生物报告基因能在不同的环境毒物下使用，O。还将三聚体型菌株改造成在微摩尔水平的铬酸盐存在下发荧光，并显示出与第一报道基因一样的特异性。它们对环境样品（加标葡萄牙河水）的适用性也证明了使用新鲜生长或冷冻保存的细胞，一种治疗，构成了操作优势。这些报告菌株可以在现场提供按需可用性，并在不久的将来可能成为一个强大的工具，在识别铬酸盐污染的网站。
Microbial bioreporters offer excellent potentialities for the detection of the bioavailable portion of pollutants in contaminated environments, which currently cannot be easily measured. This paper describes the construction and evaluation of two microbial bioreporters designed to detect the bioavailable chromate in contaminated water samples. The developed bioreporters are based on the expression of gfp under the control of the chr promoter and the chrB regulator gene of TnOtChr determinant from Ochrobactrum tritici 5bvl1. pCHRGFP1 Escherichia coli reporter proved to be specific and sensitive, with minimum detectable concentration of 100 nM chromate and did not react with other heavy metals or chemical compounds analysed. In order to have a bioreporter able to be used under different environmental toxics, O. tritici type strain was also engineered to fluoresce in the presence of micromolar levels of chromate and showed to be as specific as the first reporter. Their applicability on environmental samples (spiked Portuguese river water) was also demonstrated using either freshly grown or cryo-preserved cells, a treatment which constitutes an operational advantage. These reporter strains can provide on-demand usability in the field and in a near future may become a powerful tool in identification of chromate-contaminated sites.
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DOI：
10.3390/s100201377
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Liu X;Germaine KJ;Ryan D;Dowling DN
通讯作者：
Dowling DN
影响因子：
5.7
作者：
Magrisso S;Erel Y;Belkin S
通讯作者：
Belkin S
影响因子：
2.6
作者：
Liang, Bin;Li, Rong;Jiang, Jiandong
通讯作者：
Jiang, Jiandong
影响因子：
4.4
作者：
SELIFONOVA, O;BURLAGE, R;BARKAY, T
通讯作者：
BARKAY, T
影响因子：
3.2
作者：
Branco, Rita;Chung, Ana Paula;Zhitkovich, Anatoly
通讯作者：
Zhitkovich, Anatoly