Efficiently emissive, strongly solvatochromic and lipid droplet-specific, fluorescent probes for mapping polarity in vitro
Efficiently emissive, strongly solvatochromic and lipid droplet-specific, fluorescent probes for mapping polarity in vitro
复制标题
高效发射、强溶剂化显色和脂滴特异性荧光探针，用于体外绘制极性图
DOI：
10.1016/j.dyepig.2021.109838
复制
发表时间：
2022-01
影响因子：
4.5
通讯作者：
Lijuan Jiao
中科院分区：
文献类型：
--
作者：
Changjiang Yu;Xing Guo;Xingbao Fang;Na Chen;Qinghua Wu;Erhong Hao;Lijuan Jiao
The abnormal change of lipid droplets (LDs) polarity has been believed to be closely associated with many diseases such as fatty liver. Thus, the detection of LDs polarity plays a particular role in the diagnosis of LDs polarity-related diseases. Herein, two isoindole-based fluorescent probes have been derived and they show evident solvatochromic effects, large two-photon absorption cross-section, LDs-specificity, and high photostability and excellent performance in monitoring LDs-polarity changes in living cells. Owing to the existence of intramolecular charge transfer process, these isoindole-based probes exhibited high sensitivity between the emission wavelength maxima and solvent polarity. In spite of that, they maintained high fluorescence quantum yields up to 0.87. Moreover, the detection of LDs-polarity was demonstrated in living cellsviaconfocal laser scanning fluorescence imaging. Importantly, the isoindole-based probe maintained strong fluorescence with high contrast and clarity after seven days of degradation in a zebrafish model, indicating that it is photostable enough and suitable for long-term tracking and observation of biological dynamic changes. All these features render the isoindole chromophore an effective implement for the diagnosis of LDs polarity-related diseases.
登录
查看更多内容
影响因子：
3.5
作者：
Maria Bohnert;Bianca Schrul
通讯作者：
Maria Bohnert;Bianca Schrul
影响因子：
5.2
作者：
Peng Cao;Xiu-Li Sun;Ben-Hu Zhu;Q. Shen;Zuowei Xie;Yong Tang
通讯作者：
Peng Cao;Xiu-Li Sun;Ben-Hu Zhu;Q. Shen;Zuowei Xie;Yong Tang
有趣的“变色龙”荧光生物探针用于脂滴-溶酶体相互作用的可视化
影响因子：
14
作者：
Hu, Rong;Chen, Bin;Tang, Ben Zhong
通讯作者：
Tang, Ben Zhong
影响因子：
5.7
作者：
Kucherak, Oleksandr A.;Didier, Pascal;Klymchenko, Andrey S.
通讯作者：
Klymchenko, Andrey S.
肼-席夫碱连接双吡咯的高荧光 BF2 配合物
影响因子：
5.2
作者：
Yu Changjiang;Jiao Lijuan;Zhang Ping;Feng Zeya;Cheng Chi;Wei Yun;Mu Xiaolong;Hao Erhong
通讯作者：
Hao Erhong