Quantification of microRNA-21 and microRNA-125b in melanoma tissue
Quantification of microRNA-21 and microRNA-125b in melanoma tissue
复制标题
黑色素瘤组织中 microRNA-21 和 microRNA-125b 的定量
作者：
Anne Wandler;R. Riber‐Hansen;H. Hager;S. Hamilton;H. Schmidt;B. Nielsen;M. Stougaard;T. Steiniche
尽管 microRNA (miRNA) 已成为黑色素瘤发生和进展的有效介质，但对其致癌作用的准确理解仍不清楚。在这项研究中，我们分析了来自两个独立的黑色素瘤队列和一系列良性黑色素细胞痣的福尔马林固定和石蜡包埋的组织。使用三种不同的定量方法[阵列分析、定量 PCR (qPCR) 和通过数字图像分析定量的原位杂交 (ISH)]，我们发现肿瘤中存在相当大的 miRNA 失调。使用阵列分析，样本主要根据其生物组（良性与恶性）进行聚类，并且 77 个 miRNA 在痣和黑色素瘤样本之间存在显着差异。使用 qPCR 在两个队列中验证了黑色素瘤中 miR-21 和 miR-142 的增加以及 miR-125b、miR-211、miR-101 和 miR-513c 的减少，而使用 qPCR 无法证实通过阵列分析观察到的 miR-205 的减少。数字定量 ISH 显示黑素细胞病变中 miR-21 和 miR-125b 的表达。 miR-21 ISH 在黑色素瘤中增加，而 miR-125b 的定量显示痣和黑色素瘤中的 ISH 表达一致。我们的结果支持不同 miRNA 在黑色素瘤生物学中的重要参与，并可作为进一步研究黑色素瘤福尔马林固定和石蜡包埋组织中 miRNA 的坚实基础。特别是，与良性痣相比，黑色素瘤中 miR-21 的表达增加。
Although microRNAs (miRNAs) have emerged as potent mediators of melanoma development and progression, a precise understanding of their oncogenic role remains unclear. In this study, we analysed formalin-fixed and paraffin-embedded tissues from two separate melanoma cohorts and from a series of benign melanocytic nevi. Using three different quantification methods [array analysis, quantitative PCR (qPCR) and in-situ hybridization (ISH) quantified by digital image analysis], we found considerable miRNA dysregulation in tumours. Using array analysis, samples mainly clustered according to their biological group (benign vs. malignant) and 77 miRNAs differed significantly between nevi and melanoma samples. Increase of miR-21 and miR-142, and decrease of miR-125b, miR-211, miR-101 and miR-513c in the melanomas were verified in both cohorts using qPCR, whereas the decrease of miR-205 observed with array analysis could not be confirmed using qPCR. ISH with digital quantification showed expression of miR-21 and miR-125b in the melanocytic lesions. miR-21 ISH was increased in melanomas, whereas quantification of miR-125b showed uniform ISH expression across nevi and melanomas. Our results support the important involvement of different miRNAs in melanoma biology and may serve as solid basics for further miRNA investigations in melanoma formalin-fixed and paraffin-embedded tissue. In particular, there is increased expression of miR-21 in melanomas compared with benign nevi.
影响因子：
2.3
作者：
Latchana, Nicholas;Ganju, Akaansha;Carson, William E., III
通讯作者：
Carson, William E., III
影响因子：
16.1
作者：
Hammond SM
通讯作者：
Hammond SM
影响因子：
8
作者：
Kappelmann, M.;Kuphal, S.;Bosserhoff, A-K
通讯作者：
Bosserhoff, A-K