MicroRNA-218 Regulates Signaling Lymphocyte Activation Molecular (SLAM) Mediated Peste des Petits Ruminants Virus Infectivity in Goat Peripheral Blood Mononuclear Cells
MicroRNA-218 Regulates Signaling Lymphocyte Activation Molecular (SLAM) Mediated Peste des Petits Ruminants Virus Infectivity in Goat Peripheral Blood Mononuclear Cells
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MicroRNA-218 调节山羊外周血单核细胞中信号淋巴细胞激活分子 (SLAM) 介导的小反刍兽疫病毒感染性
DOI：
10.3389/fimmu.2019.02201
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发表时间：
2019-09
影响因子：
7.3
通讯作者：
Wang JY
中科院分区：
文献类型：
--
作者：
Qi XF;Wang T;Wang JY
Peste des petits ruminants virus (PPRV) has emerged as a significant threat to the productivity of small ruminants worldwide. SLAM was identified as the primary receptor for PPRV and other Morbilliviruses, although the regulation of SLAM expression is not yet fully understood. In this study, we revealed a novel mechanism by which PPRV upregulates its receptor SLAM expression and thereby benefits its replication via suppressing miR-218, a novel negative miRNA directly targeting SLAM gene. We demonstrated that PPRV infection downregulates miR-218, which in turn enhances SLAM expression on the surface of goat peripheral blood mononuclear cells (PBMCs), thus promoting PPRV replication. Since SLAM signaling may modulate the immune responses induced by PPRV infection, we further examined the effect of SLAM expression on the production of various cytokines by PBMCs in the absence or presence of PPRV. We demonstrated that miR-218-mediated SLAM expression modulates the expression of IFN-γ, TNF-α, and IL-10, importantly, these modulatory effects were enhanced in the presence of PPRV infection. Furthermore, our data clearly showed that PPRV H protein is sufficient to regulate miR-218-mediated SLAM expression. Taken together, our results suggest a novel mechanism involving post-transcriptional regulation of SLAM receptor expression on goat PBMCs during PPRV infection.
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影响因子：
3.7
作者：
Dhanasekaran S;Biswas M;Vignesh AR;Ramya R;Raj GD;Tirumurugaan KG;Raja A;Kataria RS;Parida S;Elankumaran S
通讯作者：
Elankumaran S
影响因子：
13.6
作者：
Trobaugh DW;Klimstra WB
通讯作者：
Klimstra WB
影响因子：
5
作者：
Manjunath, Siddappa;Mishra, Bishnu Prasad;Janga, Sarath Chandra
通讯作者：
Janga, Sarath Chandra
DOI：
10.3390/v6062287
发表时间：
2014-06-06
期刊：
Viruses
影响因子：
--
作者：
Kumar N;Maherchandani S;Kashyap SK;Singh SV;Sharma S;Chaubey KK;Ly H
通讯作者：
Ly H
影响因子：
5.4
作者：
Seki, F;Ono, N;Yanagi, Y
通讯作者：
Yanagi, Y