Expression of hedgehog proteins in the human thymus.
Expression of hedgehog proteins in the human thymus.
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DOI：
10.1177/002215540305101115
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发表时间：
2003-11
期刊：
影响因子：
--
通讯作者：
Vicente A
中科院分区：
文献类型：
--
作者：
Sacedón R;Varas A;Hernández-López C;Gutiérrez-deFrías C;Crompton T;Zapata AG;Vicente A
Hedgehog （Hh）家族的分泌蛋白包括在许多组织发育过程中指定细胞命运和模式的细胞间信号分子。在这项研究中，我们发现Hh信号通路的不同组成部分在人类胸腺中表达。三种哺乳动物Hh蛋白，Sonic (Shh), Indian （Ihh）和Desert （Dhh）是由胸腺上皮细胞产生的。表达sh的上皮细胞局限于胸腺包膜下和髓质，而产生Ihh和dhh的上皮细胞分布于整个胸腺。必要的Hh受体，Patched 1（Ptc1）和Smoothened (Smo)，以及Gli转录因子由胸腺细胞和上皮细胞表达。Ptc1在大多数胸腺细胞亚群中表达，而Smo的表达主要与未成熟胸腺细胞相关。Ptc受体的异构体Ptc2仅在胸腺内祖细胞和上皮细胞中表达。其他具有调节功能的hh结合蛋白，如刺猬相互作用蛋白（Hip）和生长抑制特异性基因1 (Gas-1)，也在人类胸腺中表达。我们的研究表明，Hh信号通路组分的胸腺内表达模式是复杂的，这表明Hh蛋白可能调节人类胸腺细胞从早期发育阶段的分化，以及胸腺上皮细胞的功能。
The Hedgehog (Hh) family of secreted proteins includes intercellular signaling molecules that specify cell fate and patterning during the development of many tissues. In this study we show that the different components of the Hh signaling pathway are expressed in human thymus. The three mammalian Hh proteins, Sonic (Shh), Indian (Ihh), and Desert (Dhh) hedgehog, are produced by thymic epithelial cells. Shh-expressing epithelial cells are restricted to the thymic subcapsula and medulla, whereas Ihh- and Dhh-producing epithelial cells are distributed throughout the thymus. The requisite Hh receptors, Patched 1(Ptc1) and Smoothened (Smo), and the Gli transcription factors are expressed by thymocytes and also by epithelial cells. Ptc1 is expressed in most thymocyte subsets, whereas Smo expression is mainly associated with immature thymocytes. The isoform of the Ptc receptor, Ptc2, is expressed only by intrathymic progenitor cells and epithelial cells. Other Hh-binding proteins with modulating functions, such as Hedgehog-interacting protein (Hip) and growth arrest-specific gene-1 (Gas-1), are also expressed in human thymus. Our study shows that the intrathymic expression pattern of the Hh signaling pathway components is complex and suggests that Hh proteins may regulate human thymocyte differentiation from the earliest developmental stages, as well as thymic epithelial cell function.
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DOI：
10.1073/pnas.93.18.9346
发表时间：
1996-09-03
影响因子：
11.1
作者：
Marigo, V;Tabin, CJ
通讯作者：
Tabin, CJ
影响因子：
56.9
作者：
LEE, JJ;EKKER, SC;BEACHY, PA
通讯作者：
BEACHY, PA
DOI：
10.1084/jem.194.2.127
发表时间：
2001-07-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lind EF;Prockop SE;Porritt HE;Petrie HT
通讯作者：
Petrie HT
影响因子：
64.5
作者：
Lewis, PM;Dunn, MP;McMahon, AP
通讯作者：
McMahon, AP
影响因子：
32.4
作者：
Outram, SV;Varas, A;Crompton, T
通讯作者：
Crompton, T