Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor.
Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor.
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DOI：
10.1186/1471-2229-14-29
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发表时间：
2014-01-14
影响因子：
5.3
通讯作者：
Iusem ND
中科院分区：
文献类型：
--
作者：
Ricardi MM;González RM;Zhong S;Domínguez PG;Duffy T;Turjanski PG;Salgado Salter JD;Alleva K;Carrari F;Giovannoni JJ;Estévez JM;Iusem ND
识别转录因子的靶基因对于解开所有类型生物体的调控网络都很重要。我们的兴趣恰恰是揭示一种广泛存在的植物转录因子调控的基因座谱，该转录因子参与对干旱的生理适应，自陆地生境400MYA殖民以来，植物就遇到了一种应激。正在研究的名为ASR1的调控因子是植物王国所独有的(尽管在拟南芥中没有)，并已知可以缓解限制供水造成的压力。由于Asr1基因的靶基因在20年前就已被克隆出来，因此我们对番茄基因组中与这一显著蛋白相互作用的特定位点进行了检测。我们使用一种高质量的抗ASR1抗体对胁迫下的番茄叶片进行了芯片和高通量DNA测序(CHIP-SEQ)。通过这种方式，我们解开了一个新的靶基因谱系，其中一些明显参与了对干旱胁迫的反应。我们发现的许多ASR1丰富的基因组基因座编码与细胞壁合成和重塑有关的酶，以及与水和溶质通量有关的通道，如水通道蛋白。此外，我们能够确定一个强大的共识ASR1结合的DNA基序。细胞壁合成和水通道蛋白基因作为ASR1的靶标的发现与它们在植物对水分损失的生理适应中的作用是一致的。这些结果深入了解了导致植物耐旱性的环境胁迫感应途径。
Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types of organisms. Our interest was precisely to uncover the spectrum of loci regulated by a widespread plant transcription factor involved in physiological adaptation to drought, a type of stress that plants have encountered since the colonization of land habitats 400 MYA. The regulator under study, named ASR1, is exclusive to the plant kingdom (albeit absent in Arabidopsis) and known to alleviate the stress caused by restricted water availability. As its target genes are still unknown despite the original cloning of Asr1 cDNA 20 years ago, we examined the tomato genome for specific loci interacting in vivo with this conspicuous protein. We performed ChIP followed by high throughput DNA sequencing (ChIP-seq) on leaves from stressed tomato plants, using a high-quality anti-ASR1 antibody. In this way, we unraveled a novel repertoire of target genes, some of which are clearly involved in the response to drought stress. Many of the ASR1-enriched genomic loci we found encode enzymes involved in cell wall synthesis and remodeling as well as channels implicated in water and solute flux, such as aquaporins. In addition, we were able to determine a robust consensus ASR1-binding DNA motif. The finding of cell wall synthesis and aquaporin genes as targets of ASR1 is consistent with their suggested role in the physiological adaptation of plants to water loss. The results gain insight into the environmental stress-sensing pathways leading to plant tolerance of drought.
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影响因子：
46.9
作者：
Ji, Hongkai;Jiang, Hui;Ma, Wenxiu;Johnson, David S.;Myers, Richard M.;Wong, Wing H.
通讯作者：
Wong, Wing H.
影响因子：
7.4
作者：
Chaumont, F;Barrieu, F;Jung, R
通讯作者：
Jung, R
影响因子：
2.7
作者：
Infante, Carlos R.;Park, Sungdae;Mihala, Alexandra G.;Kingsley, David M.;Menke, Douglas B.
通讯作者：
Menke, Douglas B.
影响因子：
7.4
作者：
Battaglia, Marina;Olvera-Carrillo, Yadira;Covarrubias, Alejandra A.
通讯作者：
Covarrubias, Alejandra A.
影响因子：
7.4
作者：
Herve, Christine;Dabos, Patrick;Tremousaygue, Dominique
通讯作者：
Tremousaygue, Dominique