Evidence of Multiple Disease Resistance (MDR) and implication of meta-analysis in marker assisted selection.
Evidence of Multiple Disease Resistance (MDR) and implication of meta-analysis in marker assisted selection.
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DOI：
10.1371/journal.pone.0068150
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yan J
中科院分区：
文献类型：
--
作者：
Ali F;Pan Q;Chen G;Zahid KR;Yan J
对玉米3种主要叶部病害进行了Meta分析，目的是找出与这些病害相关的QTL总数，并描述一些真实的QTL，为玉米分子育种和标记辅助选择（MAS）提供依据。此外，我们用一些主要的已知抗病基因和最著名的基因家族的核苷酸结合位点（NBS）编码基因证实了我们的结果。分析表明，抗病QTL在玉米基因组中随机分布，但在染色体的不同区域聚集。共检测到389个QTL，其中63个QTL控制多种病害，表现出多重抗病性。以4个QTL为标准，在基因组特定区域共检测到44个真实QTL。我们还确定了真实的QTL区域内的Ht1和Ht2基因以及14个NBS编码基因。在第8染色体上，观察到一个QTL中的两个NBS基因，在第3染色体上，观察到多个聚类和最大MDR QTL，表明明显的聚类可能是由于基因表现出多效性效应。以B73为参考基因组，病害QTL数目与每条染色体上的基因总数呈显著正相关。因此，我们认为玉米基因组中含有丰富的抗病基因，这些结果可以释放MDR现象。此外，这些结果可以非常方便地集中在不同染色体上的抗病基因和MAS精细定位的热点。
Meta-analysis was performed for three major foliar diseases with the aim to find out the total number of QTL responsible for these diseases and depict some real QTL for molecular breeding and marker assisted selection (MAS) in maize. Furthermore, we confirmed our results with some major known disease resistance genes and most well-known gene family of nucleotide binding site (NBS) encoding genes. Our analysis revealed that disease resistance QTL were randomly distributed in maize genome, but were clustered at different regions of the chromosomes. Totally 389 QTL were observed for these three major diseases in diverse maize germplasm, out of which 63 QTL were controlling more than one disease revealing the presence of multiple disease resistance (MDR). 44 real-QTLs were observed based on 4 QTL as standard in a specific region of genome. We also confirmed the Ht1 and Ht2 genes within the region of real QTL and 14 NBS-encoding genes. On chromosome 8 two NBS genes in one QTL were observed and on chromosome 3, several cluster and maximum MDR QTL were observed indicating that the apparent clustering could be due to genes exhibiting pleiotropic effect. Significant relationship was observed between the number of disease QTL and total genes per chromosome based on the reference genome B73. Therefore, we concluded that disease resistance genes are abundant in maize genome and these results can unleash the phenomenon of MDR. Furthermore, these results could be very handy to focus on hot spot on different chromosome for fine mapping of disease resistance genes and MAS.
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影响因子：
2.3
作者：
Balint-Kurti, Peter J.;Yang, Junyun;Smith, Margaret E.
通讯作者：
Smith, Margaret E.
影响因子：
5.1
作者：
Chen, CH;Chen, ZX
通讯作者：
Chen, ZX
影响因子：
5.8
作者：
Arcade, A;Labourdette, A;Joets, J
通讯作者：
Joets, J
影响因子：
5.4
作者：
BENTOLILA, S;GUITTON, C;FREYSSINET, G
通讯作者：
FREYSSINET, G
DOI：
10.1073/pnas.95.11.6531
发表时间：
1998-05-26
影响因子：
11.1
作者：
Cao, H;Li, X;Dong, XN
通讯作者：
Dong, XN