Metabolic adaptation of Ralstonia solanacearum during plant infection: a methionine biosynthesis case study.
Metabolic adaptation of Ralstonia solanacearum during plant infection: a methionine biosynthesis case study.
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植物感染期间摩纳氏菌的代谢适应：蛋氨酸生物合成案例研究。
DOI：
10.1371/journal.pone.0036877
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Genin S
中科院分区：
文献类型：
--
作者：
Plener L;Boistard P;González A;Boucher C;Genin S
MetE和MetH是两种不同的酶，其在甲硫氨酸生物合成的最后一步期间催化类似的生物化学反应，MetH是钴胺素依赖性酶，而MetE活性是钴胺素非依赖性的。在这项工作中，我们表明，在植物病原菌青枯雷尔氏菌甲硫氨酸合成的最后一步是在主致病性调节HrpG的转录控制。这种控制主要通过中间调节剂MetR对metE表达施加。metE的表达在植物细胞存在下以hrpG和metR依赖性方式被强烈且特异性地诱导。metE和metR突变体不是甲硫氨酸营养缺陷型的，并且不影响植物内的生长，但在番茄上产生显著减少的疾病症状，而metH的破坏对致病性没有影响。在植物细胞的存在下，病原体优先诱导metE表达而不是metH的发现指示了对生理宿主条件的可能的代谢适应，因为metE的这种诱导发生在其中不存在MetH所需的辅因子钴胺素的环境中。它还表明，MetE和MetH在功能上不是冗余的，并且在细菌生命周期的特定阶段部署，metE和metH的表达由多种不同的信号控制。
MetE and MetH are two distinct enzymes that catalyze a similar biochemical reaction during the last step of methionine biosynthesis, MetH being a cobalamin-dependent enzyme whereas MetE activity is cobalamin-independent. In this work, we show that the last step of methionine synthesis in the plant pathogen Ralstonia solanacearum is under the transcriptional control of the master pathogenicity regulator HrpG. This control is exerted essentially on metE expression through the intermediate regulator MetR. Expression of metE is strongly and specifically induced in the presence of plant cells in a hrpG- and metR-dependent manner. metE and metR mutants are not auxotrophic for methionine and not affected for growth inside the plant but produce significantly reduced disease symptoms on tomato whereas disruption of metH has no impact on pathogenicity. The finding that the pathogen preferentially induces metE expression rather than metH in the presence of plant cells is indicative of a probable metabolic adaptation to physiological host conditions since this induction of metE occurs in an environment in which cobalamin, the required co-factor for MetH, is absent. It also shows that MetE and MetH are not functionally redundant and are deployed during specific stages of the bacteria lifecycle, the expression of metE and metH being controlled by multiple and distinct signals.
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影响因子：
9.8
作者：
Hondorp ER;Matthews RG
通讯作者：
Matthews RG
影响因子：
3.5
作者：
Flores-Cruz, Zomary;Allen, Caitilyn
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作者：
Guo, Yinping;Figueiredo, Francisco;Wang, Nian
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作者：
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Genin, Stephane
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3.5
作者：
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