Soluble cytokine receptors are present in normal human urine.
Soluble cytokine receptors are present in normal human urine.
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DOI：
10.1084/jem.170.4.1409
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发表时间：
1989-10-01
影响因子：
15.3
通讯作者：
RUBINSTEIN, M
中科院分区：
文献类型：
--
作者：
NOVICK, D;ENGELMANN, H;WALLACH, D;RUBINSTEIN, M
在人rIL-6、人rIFN-γ或抗IFN-γ-R mAb上的粗制人尿蛋白的亲和层析产生了显著量的两种相应的可溶性受体。通过在IL-6柱和反相HPLC上通过亲和色谱串联纯化的蛋白峰的NH 2-末端微测序获得30个氨基酸残基的单一序列。该序列与先前报道的IL-6-R的预测的NH 2-末端序列相同。通过抑制固相RIA、ELISA、SDS-PAGE和Western印迹分析从IFN-γ和抗IFN-γ-R柱洗脱的蛋白质，证明正常尿液中存在可溶性IFN-γ-R。我们的发现，连同已知存在的尿TNF结合蛋白和可溶性IL-2-R在血浆和尿液中，表明可溶性细胞因子受体释放到体液中是一种普遍现象，发生在正常的生理条件下。
Affinity chromatography of crude human urinary proteins on either human rIL-6, human rIFN-gamma, or anti-IFN-gamma-R mAb yielded the two respective soluble receptors in significant quantities. A single sequence of 30 amino acid residues was obtained by NH2-terminal microsequencing of the protein peak purified in tandem by affinity chromatography on an IL-6 column and reversed-phase HPLC. This sequence was identical to the predicted NH2-terminal sequence of IL-6-R as previously reported. Analysis of the eluted proteins from both IFN- gamma and anti-IFN-gamma-R columns by inhibition of solid phase RIA, ELISA, SDS-PAGE, and Western blotting proved the existence of soluble IFN-gamma-R in normal urine. Our finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
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DOI：
10.1089/dna.1986.5.181
发表时间：
1986-06-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
MORY, Y;BENBARAK, J;REVEL, M
通讯作者：
REVEL, M
DOI：
10.1089/jir.1989.9.315
发表时间：
1989-06-01
期刊：
JOURNAL OF INTERFERON RESEARCH
影响因子：
--
作者：
NOVICK, D;FISCHER, DG;RUBINSTEIN, M
通讯作者：
RUBINSTEIN, M
影响因子：
2.9
作者：
OAKLEY, BR;KIRSCH, DR;MORRIS, NR
通讯作者：
MORRIS, NR
影响因子：
64.8
作者：
LAEMMLI, UK
通讯作者：
LAEMMLI, UK
影响因子：
15.3
作者：
PURE, E;DURIE, CJ;UNKELESS, JC
通讯作者：
UNKELESS, JC