Molecular cloning and expression in Escherichia coli of the recA gene of Legionella pneumophila.
Molecular cloning and expression in Escherichia coli of the recA gene of Legionella pneumophila.
复制标题
嗜肺军团菌recA基因的分子克隆及其在大肠杆菌中的表达。
DOI：
10.1099/00221287-135-11-3097
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Dreyfus,LA
中科院分区：
文献类型：
--
作者：
Dreyfus,LA
Interspecific complementation of anEscherichia coli recAmutant with aLegionella pneumophilagenomic library was used to identify a recombinant plasmid encoding theL. pneumophila recAgene. RecombinantE. colistrains harbouring theL. pneumophila recAgene were isolated by replica-plating bacterial colonies on medium containing methyl methanesulphonate (MMS). MMS-resistant clones were identified as encoding theL. pneumophila recAanalogue by their ability to protectE. coliHB101 from UV exposure and promote homologous recombination. Subcloning of selected restriction fragments and Tn5 mutagenesis localized therecAgene to a 1·7 kbBglII-EcoRI fragment. Analysis of minicell preparations harbouring a 1·9 kbEcoRI fragment containing therecAcoding segment revealed a single 37·5 kDa protein. Insertional inactivation of the clonedrecAgene by Tn5 resulted in the disappearance of the 37·5 kDa protein, concomitant with the loss of RecA function. TheL. pneumophila recAgene product did not promote induction of a λ lysogen; instead, the presence of the heterologousrecAgene caused a significant reduction in spontaneous and mitomycin-C-induced prophage induction inrecA+andrecA E. colibackgrounds. Despite the lack of significant genetic homology between theL. pneumophila recAgene and theE. colicounterpart, theL. pneumophilaRecA protein was nearly identical to that ofE. coliin molecular mass, and the two proteins showed antigenic cross-reactivity. Western blot analysis of UV-treatedL. pneumophilarevealed a significant increase in RecA antigen in irradiated versus control cells, suggesting that theL. pneumophila recAgene is regulated in a manner similar to that ofE. coli recA.
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影响因子：
5.4
作者：
HUGHES, SH
通讯作者：
HUGHES, SH
影响因子：
3.1
作者：
J. M. Sharma;L. Lee
通讯作者：
L. Lee
禽肉瘤病毒感染鸡的免疫状态是肉瘤生长模式和病毒抗原表达的决定因素。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Halpern,MS;McMahon,SB
通讯作者：
McMahon,SB
逆转录病毒整合的高度优选靶标
影响因子：
64.5
作者：
C. Shih;J. Stoye;J. Coffin
通讯作者：
J. Coffin
法氏囊肿瘤中禽白血病病毒 DNA 和 RNA 分析：维持肿瘤状态不需要病毒基因表达
影响因子：
64.5
作者：
G. Payne;S. Courtneidge;L. Crittenden;A. Fadly;J. Bishop;H. Varmus
通讯作者：
H. Varmus