Highly preferred targets for retrovirus integration
Highly preferred targets for retrovirus integration
复制标题
逆转录病毒整合的高度优选靶标
DOI：
10.1016/0092-8674(88)90569-7
复制
发表时间：
1988
期刊：
影响因子：
64.5
通讯作者：
J. Coffin
中科院分区：
文献类型：
--
作者：
C. Shih;J. Stoye;J. Coffin
A central feature of retrovirus replication is integration of the provirus into host cell DNA, but the specificity of this step for cell target sequences has not been clarified. To investigate this issue, we developed a method for screening and comparing large numbers of unselected integration events. Using a replicationcompetent Rous sarcoma virus containing a bacterial suppressor tRNA gene as a selectable marker, we obtained collections of clones comprising integrated provirus together with host flanking sequences. Hybridization and sequence analysis of the flanking sequence reveals the presence of a number of strongly preferred integration targets. Within these targets, independent integration events occur at sites identical to the base.
DOI：
10.1073/pnas.80.2.383
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SHIMIZU, K;GOLDFARB, M;WIGLER, M
通讯作者：
WIGLER, M
携带细菌抑制转移 RNA 基因的可行逆转录病毒基因组的构建和恢复。
DOI：
10.1126/science.2984770
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lobel,LI;Patel,M;King,W;Nguyen-Huu,MC;Goff,SP
通讯作者：
Goff,SP
通过体内重组和选择从噬菌体文库中纯化基因组序列。
影响因子：
14.9
作者：
Seed,B
通讯作者：
Seed,B