Prevalence of xenotropic murine leukaemia virus-related virus in patients with chronic fatigue syndrome in the Netherlands: retrospective analysis of samples from an established cohort.
Prevalence of xenotropic murine leukaemia virus-related virus in patients with chronic fatigue syndrome in the Netherlands: retrospective analysis of samples from an established cohort.
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DOI：
10.1136/bmj.c1018
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发表时间：
2010-02-25
期刊：
影响因子：
--
通讯作者：
van der Meer JW
中科院分区：
文献类型：
--
作者：
van Kuppeveld FJ;de Jong AS;Lanke KH;Verhaegh GW;Melchers WJ;Swanink CM;Bleijenberg G;Netea MG;Galama JM;van der Meer JW
目的报道慢性疲劳综合征患者外周血单个核细胞中存在逆转录病毒异嗜性小鼠白血病病毒相关病毒(XMRV)。考虑到这一发现潜在的巨大的医学和社会相关性，我们调查了这一发现是否可以在一个独立的欧洲慢性疲劳综合征患者队列中得到证实。聚合酶链式反应对明确队列的患者和匹配的邻里对照的设计分析。单位：荷兰一所大学医院的临床病毒学认证实验室(国际标准化组织15189)。从1991年12月至1992年4月，从76名患者和69名配对的邻里对照中分离出外周血单核细胞。在这项研究中，我们测试了32名患者和43名对照的细胞，他们的原始冷冻保存的细胞仍然可用。主要观察指标以XMRV整合酶基因为靶点的实时聚合酶链式反应和/或以XMRV gag基因为靶点的套式聚合酶链式反应检测外周血单核细胞中的XMRV。结果两种方法均未检测到XMRV序列，包括阳性分离对照、阴性分离对照和聚合酶链式反应对照。尖峰实验表明，我们能够通过实时检测和套式聚合酶链式反应检测到每105个外周血单个核细胞中至少10个拷贝的XMRV序列，表明这两种方法都具有很高的灵敏度。结论本研究未发现荷兰队列慢性疲劳综合征患者外周血单个核细胞中存在XMRV。这些数据对XMRV与大多数患者的慢性疲劳综合征有关的说法提出了质疑。
Objective The presence of the retrovirus xenotropic murine leukaemia virus-related virus (XMRV) has been reported in peripheral blood mononuclear cells of patients with chronic fatigue syndrome. Considering the potentially great medical and social relevance of such a discovery, we investigated whether this finding could be confirmed in an independent European cohort of patients with chronic fatigue syndrome. Design Analysis of a well defined cohort of patients and matched neighbourhood controls by polymerase chain reaction. Setting Certified (ISO 15189) laboratory of clinical virology in a university hospital in the Netherlands. Population Between December 1991 and April 1992, peripheral blood mononuclear cells were isolated from 76 patients and 69 matched neighbourhood controls. In this study we tested cells from 32 patients and 43 controls from whom original cryopreserved phials were still available. Main outcome measures Detection of XMRV in peripheral blood mononuclear cells by real time polymerase chain reaction assay targeting the XMRV integrase gene and/or a nested polymerase chain reaction assay targeting the XMRV gag gene. Results We detected no XMRV sequences in any of the patients or controls in either of the assays, in which relevant positive and negative isolation controls and polymerase chain reaction controls were included. Spiking experiments showed that we were able to detect at least 10 copies of XMRV sequences per 105 peripheral blood mononuclear cells by real time as well as by nested polymerase chain reaction, demonstrating high sensitivity of both assays. Conclusions This study failed to show the presence of XMRV in peripheral blood mononuclear cells of patients with chronic fatigue syndrome from a Dutch cohort. These data cast doubt on the claim that XMRV is associated with chronic fatigue syndrome in the majority of patients.
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影响因子：
3.7
作者：
Erlwein O;Kaye S;McClure MO;Weber J;Wills G;Collier D;Wessely S;Cleare A
通讯作者：
Cleare A
影响因子：
4.7
作者：
VERCOULEN, JHMM;SWANINK, CMA;BLEIJENBERG, G
通讯作者：
BLEIJENBERG, G
影响因子：
56.9
作者：
Lombardi, Vincent C.;Ruscetti, Francis W.;Mikovits, Judy A.
通讯作者：
Mikovits, Judy A.
DOI：
10.1007/s10096-008-0556-9
发表时间：
2008-12-01
影响因子：
4.5
作者：
Clancy, A.;Crowley, B.;Herra, C.
通讯作者：
Herra, C.
影响因子：
20.3
作者：
Jansen, CA;De Cuyper, IM;Miedema, F
通讯作者：
Miedema, F