Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome.
Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome.
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DOI：
10.1371/journal.pone.0008519
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发表时间：
2010-01-06
期刊：
影响因子：
3.7
通讯作者：
Cleare A
中科院分区：
文献类型：
--
作者：
Erlwein O;Kaye S;McClure MO;Weber J;Wills G;Collier D;Wessely S;Cleare A
2009年10月，据报道，美国101例慢性疲劳综合征（CFS）患者中有68例感染了一种新型γ逆转录病毒，即异嗜性小鼠白血病病毒相关病毒（XMRV），这是一种以前与前列腺癌相关的病毒。这一发现如果得到证实，将对理解和治疗一种影响全球数百万人的致残性疾病产生深远影响。我们调查了英国的CFS患者，以确定他们是否是XMRV的携带者。我们的CFS队列中的患者接受了医学筛查，以排除可检测到的器质性疾病，并符合CDC的CFS标准。从186例CFS患者的血液样本中提取的DNA进行了筛选XMRV前病毒和密切相关的小鼠白血病病毒巢式PCR使用特定的寡核苷酸引物。为了控制DNA的完整性，扩增细胞β-珠蛋白基因。包括阴性对照（水）和阳性对照（XMRV感染性分子克隆DNA）。虽然β-珠蛋白基因在所有186个样品中扩增，但既没有检测到XMRV也没有检测到MLV序列。XMRV或MLV序列未从源自英国CFS患者的DNA中扩增。虽然我们没有发现XMRV与英国CFS相关的证据，但这可能是北美和欧洲在XMRV感染的普遍患病率方面存在人口差异的结果，也可能解释了两个美国小组在前列腺癌组织中发现XMRV的事实，而两个欧洲研究没有。
In October 2009 it was reported that 68 of 101 patients with chronic fatigue syndrome (CFS) in the US were infected with a novel gamma retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), a virus previously linked to prostate cancer. This finding, if confirmed, would have a profound effect on the understanding and treatment of an incapacitating disease affecting millions worldwide. We have investigated CFS sufferers in the UK to determine if they are carriers of XMRV. Patients in our CFS cohort had undergone medical screening to exclude detectable organic illness and met the CDC criteria for CFS. DNA extracted from blood samples of 186 CFS patients were screened for XMRV provirus and for the closely related murine leukaemia virus by nested PCR using specific oligonucleotide primers. To control for the integrity of the DNA, the cellular beta-globin gene was amplified. Negative controls (water) and a positive control (XMRV infectious molecular clone DNA) were included. While the beta-globin gene was amplified in all 186 samples, neither XMRV nor MLV sequences were detected. XMRV or MLV sequences were not amplified from DNA originating from CFS patients in the UK. Although we found no evidence that XMRV is associated with CFS in the UK, this may be a result of population differences between North America and Europe regarding the general prevalence of XMRV infection, and might also explain the fact that two US groups found XMRV in prostate cancer tissue, while two European studies did not.
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DOI：
10.1046/j.1440-1614.2001.00888.x
发表时间：
2001-08-01
影响因子：
4.6
作者：
Wilson, A;Hickie, I;Lloyd, A
通讯作者：
Lloyd, A
影响因子：
56.9
作者：
Lombardi, Vincent C.;Ruscetti, Francis W.;Mikovits, Judy A.
通讯作者：
Mikovits, Judy A.
影响因子：
2.6
作者：
HONDA, M;KITAMURA, K;YAMAZAKI, S
通讯作者：
YAMAZAKI, S
影响因子：
4.1
作者：
Quarmby, Louise;Rimes, Katharine A.;Chalder, Trudie
通讯作者：
Chalder, Trudie
影响因子：
3.3
作者：
Hohn O;Krause H;Barbarotto P;Niederstadt L;Beimforde N;Denner J;Miller K;Kurth R;Bannert N
通讯作者：
Bannert N