Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration.
Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration.
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DOI：
10.1083/jcb.112.1.149
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发表时间：
1991-01
期刊：
影响因子：
--
通讯作者：
Maxfield FR
中科院分区：
文献类型：
--
作者：
Marks PW;Hendey B;Maxfield FR
细胞溶质游离钙浓度[Ca 2 +]i的瞬时增加似乎是在稀释血清存在下人嗜中性粒细胞在聚-D-赖氨酸涂覆的玻璃上迁移所必需的（Marks，P.W.，和F. R.麦克斯菲尔德1990. 110：43-52）。相反，当中性粒细胞在无血清条件下在白蛋白包被的玻璃上迁移时，不需要[Ca 2 +]i瞬变。为了确定在血清存在下聚-D-赖氨酸上需要[Ca 2 +]i瞬变的机制，在由纯化的粘附糖蛋白组成的底物上检查了迁移。在没有外部Ca 2+的情况下，导致[Ca 2 +]i瞬变停止的处理，纤连蛋白（fn）和玻连蛋白（vn）上的迁移被显著抑制。在这些底物上的Ca 2（+）缓冲细胞中，迁移也受到抑制，表明这种效应是[Ca 2 +]i改变的结果。在没有外部Ca ~（2+）的情况下，当可溶性FN或VN加入到在这些基质上迁移的细胞中时，FN或VN的迁移抑制作用更为明显。可溶性粘附糖蛋白的这种作用是特异性的：在无外界Ca 2+存在的情况下，可溶性fn不影响细胞在vn上的迁移，可溶性vn也不影响细胞在fn上的迁移。在加入可溶性粘附糖蛋白的Ca 2（+）缓冲细胞中未观察到额外的迁移抑制。这些数据表明，[Ca 2 +]i瞬变参与人中性粒细胞在fn或vn上的持续迁移，fn或vn是中性粒细胞在体内遇到的细胞外基质的一部分。
Transient increases in cytosolic free calcium concentration, [Ca2+]i, appear to be required for the migration of human neutrophils on poly-D- lysine-coated glass in the presence of dilute serum (Marks, P. W., and F. R. Maxfield. 1990. J. Cell Biol. 110:43-52). In contrast, no requirement for [Ca2+]i transients exists when neutrophils migrate on albumin-coated glass in the absence of serum. To determine the mechanism that necessitates [Ca2+]i transients on poly-D-lysine in the presence of serum, migration was examined on substrates consisting of purified adhesive glycoproteins. In the absence of external Ca2+, a treatment which causes the cessation of [Ca2+]i transients, migration on fibronectin (fn) and vitronectin (vn) was significantly inhibited. Migration was also inhibited in Ca2(+)-buffered cells on these substrates, indicating that this effect was the result of an alteration of [Ca2+]i. In the absence of external Ca2+, the inhibition of migration on fn or vn was more pronounced when soluble fn or vn was added to cells migrating on these substrates. This effect of soluble adhesive glycoprotein was specific: in the absence of external Ca2+, soluble fn did not affect the migration of cells on vn, and soluble vn did not affect the migration on fn. No additional inhibition of migration was observed in Ca2(+)-buffered cells with the addition of soluble adhesive glycoprotein. These data indicate that [Ca2+]i transients are involved in continued migration of human neutrophils on fn or vn, proteins which are part of the extracellular matrix that neutrophils encounter in vivo.
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DOI：
10.1083/jcb.110.1.43
发表时间：
1990-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Marks PW;Maxfield FR
通讯作者：
Maxfield FR
影响因子：
56.9
作者：
BOUCEK, MM;SNYDERMAN, R
通讯作者：
SNYDERMAN, R
影响因子：
15.9
作者：
ARNAOUT, MA;TODD, RF;COLTEN, HR
通讯作者：
COLTEN, HR
影响因子：
7.8
作者：
GRESHAM, HD;GOODWIN, JL;BROWN, EJ
通讯作者：
BROWN, EJ
DOI：
10.1016/0167-4889(85)90007-2
发表时间：
1985-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ELFERINK, JGR;DEIERKAUF, M
通讯作者：
DEIERKAUF, M