Tissue-specific changes in apoplastic proteins and cell wall structure during cold acclimation of winter wheat crowns.
Tissue-specific changes in apoplastic proteins and cell wall structure during cold acclimation of winter wheat crowns.
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DOI：
10.1093/jxb/erx450
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发表时间：
2018-02-23
影响因子：
6.9
通讯作者：
Tanino KK
中科院分区：
文献类型：
--
作者：
Willick IR;Takahashi D;Fowler DB;Uemura M;Tanino KK
与细胞壁修饰相关的外质体的变化以及茎尖分生组织和维管过渡区抗冻蛋白的变化表明冬小麦冠内的不同驯化反应。小麦（Triticum aestivum L.）冠是低温胁迫下过冬的关键器官。在冷适应冠中，外质体中的冰形成导致严重的组织破坏，因为它以细胞内水为代价生长。虽然以前的研究表明，维管过渡区（VTZ）比茎尖分生组织（SAM）具有更高的冻结敏感性，但其差异冻结反应背后的机制尚不完全清楚。将冷驯化的树冠冷却至-10°C，导致VTZ氯化四氮唑染色缺失，而在4°C的冷驯化21（-16°C）和42 d（-20°C）后，50%的SAM染色阳性和50%的植株恢复（LT50）的温度相似。蛋白质组学分析鉴定出脱水蛋白、春化反应蛋白和冷休克蛋白优先积聚在SAM中。相反，对VTZ的修饰集中在致病相关蛋白、抗冻蛋白和糖水解酶的增加上。傅里叶变换红外光谱焦平面阵列分析发现，细胞壁的生化修饰增强了糖醛酸糖木聚糖在VTZ中的甲基酯化交联。这些发现表明，在冷驯化过程中，SAM和VTZ表达两种不同的组织特异性外增殖反应。
Changes in the apoplast associated with cell wall modifications and anti-freeze proteins in the shoot apical meristem and vascular transition zone suggest differential acclimation responses within the winter wheat crown. The wheat (Triticum aestivum L.) crown is the critical organ of low temperature stress survival over winter. In cold-acclimated crowns, ice formation in the apoplast causes severe tissue disruption as it grows at the expense of intracellular water. While previous crown studies have shown the vascular transition zone (VTZ) to have a higher freezing sensitivity than the shoot apical meristem (SAM), the mechanism behind the differential freezing response is not fully understood. Cooling cold-acclimated crowns to –10 °C resulted in an absence of VTZ tetrazolium chloride staining, whereas the temperatures at which 50% of the SAM stained positive and 50% of plants recovered (LT50) were similar after cold acclimation for 21 (–16 °C) and 42 d (–20 °C) at 4 °C. Proteomic analysis of the apoplastic fluids identified dehydrins, vernalization-responsive proteins, and cold shock proteins preferentially accumulated in the SAM. In contrast, modifications to the VTZ centered on increases in pathogenesis-related proteins, anti-freeze proteins, and sugar hydrolyzing enzymes. Fourier transform infrared spectroscopy focal plane array analysis identified the biochemical modification of the cell wall to enhance methyl-esterified cross-linking of glucuronoarabinoxylans in the VTZ. These findings indicate that the SAM and VTZ express two distinct tissue-specific apoplastic responses during cold acclimation.
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