Systemic and mucosal immune responses following oral adenoviral delivery of influenza vaccine to the human intestine by radio controlled capsule.
Systemic and mucosal immune responses following oral adenoviral delivery of influenza vaccine to the human intestine by radio controlled capsule.
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DOI：
10.1038/srep37295
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发表时间：
2016-11-24
影响因子：
4.6
通讯作者：
Tucker SN
中科院分区：
文献类型：
--
作者：
Kim L;Martinez CJ;Hodgson KA;Trager GR;Brandl JR;Sandefer EP;Doll WJ;Liebowitz D;Tucker SN
口服免疫有几个好处，包括能够诱导粘膜免疫反应，从而保护机体免受通过粘膜表面入侵的病原体的侵袭。我们对人类免疫生物学的理解受到从人类分离粘膜细胞的困难，以及动物模型可能或可能不完全反映人类肠道免疫生物学的事实的阻碍。在这项人体药效学研究中，我们探索了一种基于腺病毒载体的新型流感血凝素表达平台。我们使用无线电控制的胶囊将疫苗运送到空肠或回肠。研究了在每个肠道部位免疫所产生的免疫反应。两个肠道部位均能诱导黏膜和全身对流感血凝素的免疫应答，但回肠输送可诱导较多的抗体分泌细胞，增加粘膜归巢B细胞和疫苗应答者的数量。总体而言，这些数据提供了对人类粘膜诱导部位的大量见解，并有助于设计和选择可用于该口服疫苗平台的适应症。
There are several benefits of oral immunization including the ability to elicit mucosal immune responses that may protect against pathogens that invade through a mucosal surface. Our understanding of human immune biology is hampered by the difficulty in isolating mucosal cells from humans, and the fact that animal models may or may not completely mirror human intestinal immunobiology. In this human pharmacodynamic study, a novel adenovirus vector-based platform expressing influenza hemagglutinin was explored. We used radio-controlled capsules to deliver the vaccine to either the jejunum or the ileum. The resulting immune responses induced by immunization at each of the intestinal sites were investigated. Both intestinal sites were capable of inducing mucosal and systemic immune responses to influenza hemagglutinin, but ileum delivery induced higher numbers of antibody secreting cells of IgG and IgA isotypes, increased mucosal homing B cells, and higher number of vaccine responders. Overall, these data provided substantial insights into human mucosal inductive sites, and aided in the design and selection of indications that could be used with this oral vaccine platform.
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DOI：
10.1084/jem.20010670
发表时间：
2002-01-21
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bowman EP;Kuklin NA;Youngman KR;Lazarus NH;Kunkel EJ;Pan J;Greenberg HB;Butcher EC
通讯作者：
Butcher EC
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
5.5
作者：
Gordon DL;Sajkov D;Woodman RJ;Honda-Okubo Y;Cox MM;Heinzel S;Petrovsky N
通讯作者：
Petrovsky N
影响因子：
4.9
作者：
Van Kruiningen, HJ;West, AB;Holmes, KA
通讯作者：
Holmes, KA
影响因子：
7.3
作者：
Kaminski DA;Wei C;Qian Y;Rosenberg AF;Sanz I
通讯作者：
Sanz I