OsYSL13 Is Involved in Iron Distribution in Rice.

OsYSL13 Is Involved in Iron Distribution in Rice.
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OsYSL13 参与水稻中的铁分配

DOI:
10.3390/ijms19113537
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发表时间:
2018-11-09
影响因子:
5.6
通讯作者:
Zheng L
Zheng L
中科院分区:
生物学2区
文献类型:
--
作者:
Zhang C;Shinwari KI;Luo L;Zheng L

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植物对铁的吸收和转运对植物生长和人类健康都具有重要意义。然而,关于植物,特别是农作物中铁的运输机制却知之甚少。本研究分析了水稻黄条样蛋白13(YSL 13)的功能。OsYSL 13在叶片中表达量较高,尤其是在叶片中,而在根和地上部中的表达均受缺铁诱导。此外,OsYSL 13在老叶中的表达水平高于幼叶。OsYSL 13定位于细胞质膜。金属测量结果表明,铁浓度较低,在最年轻的叶片和较高的老叶的osysl13突变体铁充足和不足的条件下,与野生型和两个互补线。此外,铁的浓度在糙米和种子的osysl13突变体也降低。在OsYSL13过表达株系中发现了相反的结果。这些结果表明OsYSL13参与了水稻中Fe的分布。
The uptake and transport of iron (Fe) in plants are both important for plant growth and human health. However, little is known about the mechanism of Fe transport in plants, especially for crops. In the present study, the function of yellow stripe-like 13 (YSL13) in rice was analyzed. OsYSL13 was highly expressed in leaves, especially in leaf blades, whereas its expression was induced by Fe deficiency both in roots and shoots. Furthermore, the expression level of OsYSL13 was higher in older leaves than that in younger leaves. OsYSL13 was located in the plasma membrane. Metal measurement revealed that Fe concentrations were lower in the youngest leaf and higher in the older leaves of the osysl13 mutant under both Fe sufficiency and deficiency conditions, compared with the wild type and two complementation lines. Moreover, the Fe concentrations in the brown rice and seeds of the osysl13 mutant were also reduced. Opposite results were found in OsYSL13 overexpression lines. These results suggest that OsYSL13 is involved in Fe distribution in rice.
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